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题名: Hydrophilic interaction/weak cation-exchange mixed-mode chromatography for chitooligosaccharides separation
作者: Dong, Xuefang1;  Shen, Aijin1;  Gou, Zhimou1;  Chen, Deliang2;  Liang, Xinmiao1
关键词: Chitooligosaccharides ;  Weak cation-exchange chromatography ;  Hydrophilic interaction chromatography ;  Mixed-mode chromatography
刊名: CARBOHYDRATE RESEARCH
发表日期: 2012-11-01
DOI: 10.1016/j.carres.2012.08.022
卷: 361, 页:195-199
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemistry & Molecular Biology ;  Chemistry, Applied ;  Chemistry, Organic
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A hydrophilic interaction/weak cation-exchange (HILIC/WCX) mixed-mode chromatography was developed for separation of chitooligosaccharides (COS) using a weak cation-exchange column. For better understanding of the retention characteristics of the column, organic solvent content effect was investigated. WCX mode, HILIC mode, and mixed-mode could be performed on this column according to investigating results. COS were separated on the column in mixed-mode. The usage of volatile buffer salt made it favorable for evaporative light-scattering detector (ELSD). Mild elution conditions were economical and environment-friendly relative to other conditions used in early reports. This method may offer a possibility for large-scale COS preparation. (c) 2012 Elsevier Ltd. All rights reserved.
关键词[WOS]: NUCLEIC-ACIDS ;  CHITOSAN ;  OLIGOSACCHARIDES ;  OLIGOMERS ;  PEPTIDES
语种: 英语
WOS记录号: WOS:000310843700028
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/143163
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Wuhan Univ, ZhongNan Hosp, Dept Internal Med, Div Cardiol, Wuhan 430071, Hubei Province, Peoples R China

Recommended Citation:
Dong, Xuefang,Shen, Aijin,Gou, Zhimou,et al. Hydrophilic interaction/weak cation-exchange mixed-mode chromatography for chitooligosaccharides separation[J]. CARBOHYDRATE RESEARCH,2012,361:195-199.
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