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题名: A highly selective ratiometric fluorescent probe for in vitro monitoring and cellular imaging of human carboxylesterase 1
作者: Liu, Zhao-Ming1;  Feng, Lei2;  Ge, Guang-Bo1;  Lv, Xia1;  Hou, Jie3;  Cao, Yun-Feng1;  Cui, Jing-Nan2;  Yang, Ling1
关键词: Ratiometric fluorescent probe ;  Human carboxylesterase 1 ;  Large emission shift ;  Cell imaging
刊名: BIOSENSORS & BIOELECTRONICS
发表日期: 2014-07-15
DOI: 10.1016/j.bios.2014.01.049
卷: 57, 页:30-35
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biophysics ;  Biotechnology & Applied Microbiology ;  Chemistry, Analytical ;  Electrochemistry ;  Nanoscience & Nanotechnology
研究领域[WOS]: Biophysics ;  Biotechnology & Applied Microbiology ;  Chemistry ;  Electrochemistry ;  Science & Technology - Other Topics
英文摘要: A new ratiometric fluorescent probe derived from 2-(2-hydroxy-3-methoxyphenyl) benzothiazole (HMBT) has been developed for selective monitoring of human carboxylesterase 1 (hCE1). The probe is designed by introducing benzoyl moiety to HMBT. The prepared latent spectroscopic probe 1 displays satisfying stability under physiological pH conditions with very low background signal. Both the reaction phynotyping and chemical inhibition assays demonstrated that hCE1 mediated the specific cleavage of the carboxylic ester bond of probe 1 in human biological samples. The release of HMBT leads to a remarkable red-shifted emission in fluorescence spectrum (120 nm large emission shift). Furthermore, human cell-based assays show that probe 1 is cell membrane permeable, and it can be used for bioassay and cellular imaging of hCE1 activity in HepG2 cells. These findings lead to the development of a simple and sensitive fluorescent method for measurement of hCE1 activity in vitro or in living cells, in the presence of additional enzymes or endogenous compounds. (C) 2014 Elsevier B.V. All rights reserved.
关键词[WOS]: ESTER HYDROLASE 1 ;  HUMAN LIVER ;  MAMMALIAN CARBOXYLESTERASES ;  DRUG-METABOLISM ;  LIVING CELLS ;  HYDROLYSIS ;  CLOPIDOGREL ;  BINDING ;  OSELTAMIVIR ;  ACTIVATION
语种: 英语
WOS记录号: WOS:000334086100005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/143176
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China
2.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China
3.Dalian Med Univ, Dalian 116044, Peoples R China

Recommended Citation:
Liu, Zhao-Ming,Feng, Lei,Ge, Guang-Bo,et al. A highly selective ratiometric fluorescent probe for in vitro monitoring and cellular imaging of human carboxylesterase 1[J]. BIOSENSORS & BIOELECTRONICS,2014,57:30-35.
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