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题名: Evaluation and comparison of n-alkyl chain and polar ligand bonded stationary phases for protein separation in reversed-phase liquid chromatography
作者: Ding, Ling1;  Guo, Zhimou1;  Xiao, Yuansheng1;  Xue, Xingya1;  Zhang, Xiuli1;  Liang, Xinmiao1
关键词: Alkyl chains ;  Polar ligands ;  Protein separation ;  Retention equations ;  Reversed-phase liquid chromatography
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2014-09-01
DOI: 10.1002/jssc.201400238
卷: 37, 期:18, 页:2467-2473
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Protein retention is very sensitive to the change of solvent composition in reversed-phase liquid chromatography for so called "on-off" mechanism, leading to difficulty in mobile phase optimization. In this study, a novel 3-chloropropyl trichlorosilane ligand bonded column was prepared for protein separation. The differences in retention characteristics between t he 3-chloropropyl trichlorosilane ligand bonded column and n-alkyl chainmodified (C-2, C-4, C-8) stationary phases were elucidated by the retention equation ln k = a + cC(B). Retention parameters (a and c) of nine standard proteins with different molecular weights were calculated by using homemade software. Results showed that retention times of nine proteins were similar on four columns, but the 3-chloropropyl trichlorosilane ligand bonded column obtained the lowest retention parameter values of larger proteins. It meant that their retention behavior affected by acetonitrile concentration would be different due to lower vertical bar c vertical bar values. More specifically, protein elution windows were broader, and retentions were less sensitive to the change of acetonitrile concentration on the 3-chloropropyl trichlorosilane ligand bonded column than that on other columns. Meanwhile, the 3-chloropropyl trichlorosilane ligand bonded column displayed distinctive selectivity for some proteins. Our results indicated that stationary phase with polar ligand provided potential solutions to the "on-off" problem and optimization in protein separation.
关键词[WOS]: GRADIENT ELUTION ;  HORIZONTAL POLYMERIZATION ;  EXCHANGE CHROMATOGRAPHY ;  RETENTION EQUATION ;  INTACT PROTEINS ;  PERFORMANCE ;  SILICA ;  PEPTIDES ;  PACKINGS ;  HPLC
语种: 英语
WOS记录号: WOS:000342909000002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/143196
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Ding, Ling,Guo, Zhimou,Xiao, Yuansheng,et al. Evaluation and comparison of n-alkyl chain and polar ligand bonded stationary phases for protein separation in reversed-phase liquid chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2014,37(18):2467-2473.
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