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Evaluation and comparison of n-alkyl chain and polar ligand bonded stationary phases for protein separation in reversed-phase liquid chromatography
Ding, Ling; Guo, Zhimou; Xiao, Yuansheng; Xue, Xingya; Zhang, Xiuli; Liang, Xinmiao
关键词Alkyl Chains Polar Ligands Protein Separation Retention Equations Reversed-phase Liquid Chromatography
刊名JOURNAL OF SEPARATION SCIENCE
2014-09-01
DOI10.1002/jssc.201400238
37期:18页:2467-2473
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]GRADIENT ELUTION ; HORIZONTAL POLYMERIZATION ; EXCHANGE CHROMATOGRAPHY ; RETENTION EQUATION ; INTACT PROTEINS ; PERFORMANCE ; SILICA ; PEPTIDES ; PACKINGS ; HPLC
英文摘要Protein retention is very sensitive to the change of solvent composition in reversed-phase liquid chromatography for so called "on-off" mechanism, leading to difficulty in mobile phase optimization. In this study, a novel 3-chloropropyl trichlorosilane ligand bonded column was prepared for protein separation. The differences in retention characteristics between t he 3-chloropropyl trichlorosilane ligand bonded column and n-alkyl chainmodified (C-2, C-4, C-8) stationary phases were elucidated by the retention equation ln k = a + cC(B). Retention parameters (a and c) of nine standard proteins with different molecular weights were calculated by using homemade software. Results showed that retention times of nine proteins were similar on four columns, but the 3-chloropropyl trichlorosilane ligand bonded column obtained the lowest retention parameter values of larger proteins. It meant that their retention behavior affected by acetonitrile concentration would be different due to lower vertical bar c vertical bar values. More specifically, protein elution windows were broader, and retentions were less sensitive to the change of acetonitrile concentration on the 3-chloropropyl trichlorosilane ligand bonded column than that on other columns. Meanwhile, the 3-chloropropyl trichlorosilane ligand bonded column displayed distinctive selectivity for some proteins. Our results indicated that stationary phase with polar ligand provided potential solutions to the "on-off" problem and optimization in protein separation.
语种英语
WOS记录号WOS:000342909000002
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/143196
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
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GB/T 7714
Ding, Ling,Guo, Zhimou,Xiao, Yuansheng,et al. Evaluation and comparison of n-alkyl chain and polar ligand bonded stationary phases for protein separation in reversed-phase liquid chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2014,37(18):2467-2473.
APA Ding, Ling,Guo, Zhimou,Xiao, Yuansheng,Xue, Xingya,Zhang, Xiuli,&Liang, Xinmiao.(2014).Evaluation and comparison of n-alkyl chain and polar ligand bonded stationary phases for protein separation in reversed-phase liquid chromatography.JOURNAL OF SEPARATION SCIENCE,37(18),2467-2473.
MLA Ding, Ling,et al."Evaluation and comparison of n-alkyl chain and polar ligand bonded stationary phases for protein separation in reversed-phase liquid chromatography".JOURNAL OF SEPARATION SCIENCE 37.18(2014):2467-2473.
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