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Evaluation and comparison of n-alkyl chain and polar ligand bonded stationary phases for protein separation in reversed-phase liquid chromatography
Ding, Ling; Guo, Zhimou; Xiao, Yuansheng; Xue, Xingya; Zhang, Xiuli; Liang, Xinmiao
KeywordAlkyl Chains Polar Ligands Protein Separation Retention Equations Reversed-phase Liquid Chromatography
Source PublicationJOURNAL OF SEPARATION SCIENCE
2014-09-01
DOI10.1002/jssc.201400238
Volume37Issue:18Pages:2467-2473
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordGRADIENT ELUTION ; HORIZONTAL POLYMERIZATION ; EXCHANGE CHROMATOGRAPHY ; RETENTION EQUATION ; INTACT PROTEINS ; PERFORMANCE ; SILICA ; PEPTIDES ; PACKINGS ; HPLC
AbstractProtein retention is very sensitive to the change of solvent composition in reversed-phase liquid chromatography for so called "on-off" mechanism, leading to difficulty in mobile phase optimization. In this study, a novel 3-chloropropyl trichlorosilane ligand bonded column was prepared for protein separation. The differences in retention characteristics between t he 3-chloropropyl trichlorosilane ligand bonded column and n-alkyl chainmodified (C-2, C-4, C-8) stationary phases were elucidated by the retention equation ln k = a + cC(B). Retention parameters (a and c) of nine standard proteins with different molecular weights were calculated by using homemade software. Results showed that retention times of nine proteins were similar on four columns, but the 3-chloropropyl trichlorosilane ligand bonded column obtained the lowest retention parameter values of larger proteins. It meant that their retention behavior affected by acetonitrile concentration would be different due to lower vertical bar c vertical bar values. More specifically, protein elution windows were broader, and retentions were less sensitive to the change of acetonitrile concentration on the 3-chloropropyl trichlorosilane ligand bonded column than that on other columns. Meanwhile, the 3-chloropropyl trichlorosilane ligand bonded column displayed distinctive selectivity for some proteins. Our results indicated that stationary phase with polar ligand provided potential solutions to the "on-off" problem and optimization in protein separation.
Language英语
WOS IDWOS:000342909000002
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/143196
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Ding, Ling,Guo, Zhimou,Xiao, Yuansheng,et al. Evaluation and comparison of n-alkyl chain and polar ligand bonded stationary phases for protein separation in reversed-phase liquid chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2014,37(18):2467-2473.
APA Ding, Ling,Guo, Zhimou,Xiao, Yuansheng,Xue, Xingya,Zhang, Xiuli,&Liang, Xinmiao.(2014).Evaluation and comparison of n-alkyl chain and polar ligand bonded stationary phases for protein separation in reversed-phase liquid chromatography.JOURNAL OF SEPARATION SCIENCE,37(18),2467-2473.
MLA Ding, Ling,et al."Evaluation and comparison of n-alkyl chain and polar ligand bonded stationary phases for protein separation in reversed-phase liquid chromatography".JOURNAL OF SEPARATION SCIENCE 37.18(2014):2467-2473.
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