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Dendrimer-grafted graphene oxide nanosheets as novel support for trypsin immobilization to achieve fast on-plate digestion of proteins
Jiang, Bo1,2,3; Yang, Kaiguang2; Zhang, Lihua2; Liang, Zhen2; Peng, Xiaojun1; Zhang, Yukui2
KeywordGraphene Oxide Dendrimer On-plate Digestion Proteomics Imer
Source PublicationTALANTA
2014-05-01
DOI10.1016/j.talanta.2014.01.056
Volume122Pages:278-284
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordMALDI-TOF-MS ; ENTRAPPED GOLD NANOPARTICLES ; EFFICIENT PROTEOME DIGESTION ; MAGNETIC NANOPARTICLES ; IMAGING APPLICATIONS ; DRUG-DELIVERY ; PROTEOLYSIS ; HYBRID ; NANOCOMPOSITES ; IDENTIFICATION
AbstractIn this study, dendrimer grafted graphene oxide nanosheets (dGO) were prepared by covalent reaction. The successful synthesis of dGO was confirmed by Fourier-transform infrared spectra, Raman spectra, Thermo gravimetric analysis and Zeta potential. Taking advantages of large surface area, excellent biocompatibility and abundant functional groups, dGO provided an ideal substrate for trypsin immobilization. Trypsin-linked dGO was synthesized through covalent bonding using glutaraldehyde as coupling agents. The amount of trypsin immobilized on dGO nanosheets was calculated to be about 649 + 20 mg/ g. The activity of immobilized trypsin could be maintained for over 10 days at 4 C. On-plate proteolysis could be performed without removing trypsin-linked dGO, because dGO did not interfere with matrixassisted laser desorption ionization time-of-flight tandem mass spectrometry analysis. By such an immobilized enzymatic reactor, standard proteins could be efficiently digested within 15 min, with sequence coverages comparable or better than those obtained by conventional over-night in-solution digestion. Furthermore, trypsin-linked dGO showed high sensitivity when applied to trace samples analysis. All these results demonstrated that the developed dGO based enzymatic reactor might provide a promising tool for high throughput proteome identification. (C) 2014 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000335636700043
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/143219
Collection中国科学院大连化学物理研究所
Affiliation1.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Jiang, Bo,Yang, Kaiguang,Zhang, Lihua,et al. Dendrimer-grafted graphene oxide nanosheets as novel support for trypsin immobilization to achieve fast on-plate digestion of proteins[J]. TALANTA,2014,122:278-284.
APA Jiang, Bo,Yang, Kaiguang,Zhang, Lihua,Liang, Zhen,Peng, Xiaojun,&Zhang, Yukui.(2014).Dendrimer-grafted graphene oxide nanosheets as novel support for trypsin immobilization to achieve fast on-plate digestion of proteins.TALANTA,122,278-284.
MLA Jiang, Bo,et al."Dendrimer-grafted graphene oxide nanosheets as novel support for trypsin immobilization to achieve fast on-plate digestion of proteins".TALANTA 122(2014):278-284.
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