DICP OpenIR
Dendrimer-grafted graphene oxide nanosheets as novel support for trypsin immobilization to achieve fast on-plate digestion of proteins
Jiang, Bo1,2,3; Yang, Kaiguang2; Zhang, Lihua2; Liang, Zhen2; Peng, Xiaojun1; Zhang, Yukui2
关键词Graphene Oxide Dendrimer On-plate Digestion Proteomics Imer
刊名TALANTA
2014-05-01
DOI10.1016/j.talanta.2014.01.056
122页:278-284
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]MALDI-TOF-MS ; ENTRAPPED GOLD NANOPARTICLES ; EFFICIENT PROTEOME DIGESTION ; MAGNETIC NANOPARTICLES ; IMAGING APPLICATIONS ; DRUG-DELIVERY ; PROTEOLYSIS ; HYBRID ; NANOCOMPOSITES ; IDENTIFICATION
英文摘要In this study, dendrimer grafted graphene oxide nanosheets (dGO) were prepared by covalent reaction. The successful synthesis of dGO was confirmed by Fourier-transform infrared spectra, Raman spectra, Thermo gravimetric analysis and Zeta potential. Taking advantages of large surface area, excellent biocompatibility and abundant functional groups, dGO provided an ideal substrate for trypsin immobilization. Trypsin-linked dGO was synthesized through covalent bonding using glutaraldehyde as coupling agents. The amount of trypsin immobilized on dGO nanosheets was calculated to be about 649 + 20 mg/ g. The activity of immobilized trypsin could be maintained for over 10 days at 4 C. On-plate proteolysis could be performed without removing trypsin-linked dGO, because dGO did not interfere with matrixassisted laser desorption ionization time-of-flight tandem mass spectrometry analysis. By such an immobilized enzymatic reactor, standard proteins could be efficiently digested within 15 min, with sequence coverages comparable or better than those obtained by conventional over-night in-solution digestion. Furthermore, trypsin-linked dGO showed high sensitivity when applied to trace samples analysis. All these results demonstrated that the developed dGO based enzymatic reactor might provide a promising tool for high throughput proteome identification. (C) 2014 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000335636700043
引用统计
被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/143219
专题中国科学院大连化学物理研究所
作者单位1.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714
Jiang, Bo,Yang, Kaiguang,Zhang, Lihua,et al. Dendrimer-grafted graphene oxide nanosheets as novel support for trypsin immobilization to achieve fast on-plate digestion of proteins[J]. TALANTA,2014,122:278-284.
APA Jiang, Bo,Yang, Kaiguang,Zhang, Lihua,Liang, Zhen,Peng, Xiaojun,&Zhang, Yukui.(2014).Dendrimer-grafted graphene oxide nanosheets as novel support for trypsin immobilization to achieve fast on-plate digestion of proteins.TALANTA,122,278-284.
MLA Jiang, Bo,et al."Dendrimer-grafted graphene oxide nanosheets as novel support for trypsin immobilization to achieve fast on-plate digestion of proteins".TALANTA 122(2014):278-284.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Jiang, Bo]的文章
[Yang, Kaiguang]的文章
[Zhang, Lihua]的文章
百度学术
百度学术中相似的文章
[Jiang, Bo]的文章
[Yang, Kaiguang]的文章
[Zhang, Lihua]的文章
必应学术
必应学术中相似的文章
[Jiang, Bo]的文章
[Yang, Kaiguang]的文章
[Zhang, Lihua]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。