中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
学科主题: 物理化学
题名: Establishment of a new OSCC cell line derived from OLK and identification of malignant transformation-related proteins by differential proteomics approach
作者: Dong, Yan1, 2, 3;  Zhao, Qun4;  Ma, Xiaoyan1, 2, 3;  Ma, Guowu1;  Liu, Caiyun1, 2, 3;  Chen, Zhuwen1;  Yu, Liyuan1, 2, 3;  Liu, Xuefeng5;  Zhang, Yanguang1, 2, 3;  Shao, Shujuan5;  Xiao, Jing1;  Li, Jia2, 3;  Zhang, Weimin2, 3;  Fu, Ming2, 3;  Dong, Lijia2, 3;  Yang, Xiandong1;  Guo, Xu6;  Xue, Liyan3, 7, 8;  Fang, Fei4;  Zhan, Qimin2, 3;  Zhang, Lihua4
通讯作者: YanDong ;  赵群
刊名: SCIENTIFIC REPORTS
发表日期: 2015-08-03
DOI: 10.1038/srep12668
卷: 5, 期:1, 页:1
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
英文摘要: Oral squamous cell carcinoma (OSCC) is usually preceded by the oral premalignant lesions, mainly oral leukoplakia (OLK) after repeated insults of carcinogens, tobacco. B(a)P and DMBA are key carcinogens in tobacco smoke. In the present study, for the first time we established the cancerous cell line OSCC-BD induced by B(a)P/DMBA mixture and transformed from dysplastic oral leukoplakia cell line DOK. Cell morphology, proliferation ability, migration ability, colony formation, and tumorigenicity were studied and confirmed the malignant characteristics of OSCC-BD cells. We further identified the differential proteins between DOK and OSCC-BD cells by stable isotope dimethyl labeling based quantitative proteomic method, which showed 18 proteins up-regulated and 16 proteins down-regulated with RSD < 8%. Differential proteins are mainly related to cell cycle, cell proliferation, DNA replication, RNA splicing and apoptosis. Abberant binding function, catalysis activity and transportor activity of differential proteins might contribute to the malignant transformation of OLK. Of the 34 identified differential proteins with RSD < 8%, 13 novel cancer-related proteins were reported in the present study. This study might provide a new insight into the mechanism of OLK malignant transformation and the potent biomarkers for early diagnosis, meanwhile further facilitate the application of the quantification proteomics to carcinogenesis research.
关键词[WOS]: BREAST-CANCER ;  MCM4 EXPRESSION ;  CARCINOMA ;  OVEREXPRESSION ;  QUANTIFICATION ;  CARCINOGENESIS ;  INCREASE ;  MARKER
语种: 英语
WOS记录号: WOS:000358856000001
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/143688
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
2015gFeQrdxdzW.PDF(1126KB)期刊论文作者接受稿开放获取View Download

作者单位: 1.Dalian Med Univ, Coll Stomatol, Dalian 116044, Peoples R China
2.Chinese Acad Med Sci, State Key Lab Mol Oncol, Beijing 100021, Peoples R China
3.Peking Union Med Coll, Beijing 100021, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
5.Dalian Med Univ, Affiliated Hosp 2, Ctr Canc, Inst Canc Stem Cell, Dalian 116044, Peoples R China
6.Dalian Univ Technol, Dept Engn Mech, Dalian 116023, Peoples R China
7.Chinese Acad Med Sci, Canc Hosp, Dept Pathol, Beijing 100021, Peoples R China
8.Chinese Acad Med Sci, Inst Canc, Beijing 100021, Peoples R China

Recommended Citation:
Dong, Yan,Zhao, Qun,Ma, Xiaoyan,et al. Establishment of a new OSCC cell line derived from OLK and identification of malignant transformation-related proteins by differential proteomics approach[J]. SCIENTIFIC REPORTS,2015,5(1):1.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Dong, Yan]'s Articles
 [Zhao, Qun]'s Articles
 [Ma, Xiaoyan]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Dong, Yan]‘s Articles
 [Zhao, Qun]‘s Articles
 [Ma, Xiaoyan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
文件名: 2015gFeQrdxdzW.PDF
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace