DICP OpenIR
Subject Area物理化学
Establishment of a new OSCC cell line derived from OLK and identification of malignant transformation-related proteins by differential proteomics approach
Dong, Yan1,2,3; Zhao, Qun4; Ma, Xiaoyan1,2,3; Ma, Guowu1; Liu, Caiyun1,2,3; Chen, Zhuwen1; Yu, Liyuan1,2,3; Liu, Xuefeng5; Zhang, Yanguang1,2,3; Shao, Shujuan5; Xiao, Jing1; Li, Jia2,3; Zhang, Weimin2,3; Fu, Ming2,3; Dong, Lijia2,3; Yang, Xiandong1; Guo, Xu6; Xue, Liyan3,7,8; Fang, Fei4; Zhan, Qimin2,3; Zhang, Lihua4; YanDong; Zhao Q(赵群)
Source PublicationSCIENTIFIC REPORTS
2015-08-03
DOI10.1038/srep12668
Volume5Issue:1Pages:1
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology
WOS SubjectMultidisciplinary Sciences
WOS Research AreaScience & Technology - Other Topics
WOS KeywordBREAST-CANCER ; MCM4 EXPRESSION ; CARCINOMA ; OVEREXPRESSION ; QUANTIFICATION ; CARCINOGENESIS ; INCREASE ; MARKER
AbstractOral squamous cell carcinoma (OSCC) is usually preceded by the oral premalignant lesions, mainly oral leukoplakia (OLK) after repeated insults of carcinogens, tobacco. B(a)P and DMBA are key carcinogens in tobacco smoke. In the present study, for the first time we established the cancerous cell line OSCC-BD induced by B(a)P/DMBA mixture and transformed from dysplastic oral leukoplakia cell line DOK. Cell morphology, proliferation ability, migration ability, colony formation, and tumorigenicity were studied and confirmed the malignant characteristics of OSCC-BD cells. We further identified the differential proteins between DOK and OSCC-BD cells by stable isotope dimethyl labeling based quantitative proteomic method, which showed 18 proteins up-regulated and 16 proteins down-regulated with RSD < 8%. Differential proteins are mainly related to cell cycle, cell proliferation, DNA replication, RNA splicing and apoptosis. Abberant binding function, catalysis activity and transportor activity of differential proteins might contribute to the malignant transformation of OLK. Of the 34 identified differential proteins with RSD < 8%, 13 novel cancer-related proteins were reported in the present study. This study might provide a new insight into the mechanism of OLK malignant transformation and the potent biomarkers for early diagnosis, meanwhile further facilitate the application of the quantification proteomics to carcinogenesis research.
Language英语
WOS IDWOS:000358856000001
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/143688
Collection中国科学院大连化学物理研究所
Corresponding AuthorYanDong; Zhao Q(赵群)
Affiliation1.Dalian Med Univ, Coll Stomatol, Dalian 116044, Peoples R China
2.Chinese Acad Med Sci, State Key Lab Mol Oncol, Beijing 100021, Peoples R China
3.Peking Union Med Coll, Beijing 100021, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
5.Dalian Med Univ, Affiliated Hosp 2, Ctr Canc, Inst Canc Stem Cell, Dalian 116044, Peoples R China
6.Dalian Univ Technol, Dept Engn Mech, Dalian 116023, Peoples R China
7.Chinese Acad Med Sci, Canc Hosp, Dept Pathol, Beijing 100021, Peoples R China
8.Chinese Acad Med Sci, Inst Canc, Beijing 100021, Peoples R China
Recommended Citation
GB/T 7714
Dong, Yan,Zhao, Qun,Ma, Xiaoyan,et al. Establishment of a new OSCC cell line derived from OLK and identification of malignant transformation-related proteins by differential proteomics approach[J]. SCIENTIFIC REPORTS,2015,5(1):1.
APA Dong, Yan.,Zhao, Qun.,Ma, Xiaoyan.,Ma, Guowu.,Liu, Caiyun.,...&赵群.(2015).Establishment of a new OSCC cell line derived from OLK and identification of malignant transformation-related proteins by differential proteomics approach.SCIENTIFIC REPORTS,5(1),1.
MLA Dong, Yan,et al."Establishment of a new OSCC cell line derived from OLK and identification of malignant transformation-related proteins by differential proteomics approach".SCIENTIFIC REPORTS 5.1(2015):1.
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