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学科主题物理化学
Quantitative characterization of histone post-translational modifications using a stable isotope dimethyl-labeling strategy
Han, Yanpu1; Lu, Congcong1; Zhang, Kai1,2,3; Tian, Shanshan2,3; Fan, Enguo4; Chen, Langxing1; He, Xiwen1; Zhang, Yukui1,5; Zhang K(张锴)
刊名ANALYTICAL METHODS
2015
DOI10.1039/c5ay00120j
7期:9页:3779-3785
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine ; Technology
类目[WOS]Chemistry, Analytical ; Food Science & Technology ; Spectroscopy
研究领域[WOS]Chemistry ; Food Science & Technology ; Spectroscopy
关键词[WOS]METHYLATION ; PROTEOMICS ; LANGUAGE ; MARKS
英文摘要Histone post-translational modifications (PTMs) have been considered to be a major group of important epigenetic marks and play critical roles in the regulation of chromatin-templated biological processes. To date, novel strategies for the quantification of histone PTMs are still highly desirable. Herein, we present an efficient approach to quantitatively characterize histone PTMs using stable isotope dimethyl labeling coupled with mass spectrometry. At first, all of the 3-aminogroups of free lysines are derivatized by heavy formaldehyde to enable an easy distinction of free lysines from those of naturally occurring lysine-dimethylation upon MS analysis. After tryptic digestion, a second derivatization was applied with heavy- and light-stable isotope dimethyl labeling to label the N-termini of tryptic peptides from different sample sources. The mixture was further identified and quantified by HPLC-MS/MS. This method enables the comparison of histone PTMs from multiple sample sources and the quantification of different PTMs at certain amino-acid residues of histones in one single experiment. Thus, it is highly attractive for the identification of epigenetic histone marks.
语种英语
WOS记录号WOS:000354042300020
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/143692
专题中国科学院大连化学物理研究所
通讯作者Zhang K(张锴)
作者单位1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Tianjin Med Univ, Tianjin Key Lab Med Epigenet, Tianjin 300070, Peoples R China
3.Tianjin Med Univ, Sch Basic Med Sci, Tianjin 300070, Peoples R China
4.Univ Freiburg, Inst Biochem & Mol Biol, ZBMZ, D-79104 Freiburg, Germany
5.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
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Han, Yanpu,Lu, Congcong,Zhang, Kai,et al. Quantitative characterization of histone post-translational modifications using a stable isotope dimethyl-labeling strategy[J]. ANALYTICAL METHODS,2015,7(9):3779-3785.
APA Han, Yanpu.,Lu, Congcong.,Zhang, Kai.,Tian, Shanshan.,Fan, Enguo.,...&张锴.(2015).Quantitative characterization of histone post-translational modifications using a stable isotope dimethyl-labeling strategy.ANALYTICAL METHODS,7(9),3779-3785.
MLA Han, Yanpu,et al."Quantitative characterization of histone post-translational modifications using a stable isotope dimethyl-labeling strategy".ANALYTICAL METHODS 7.9(2015):3779-3785.
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