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学科主题: 物理化学
题名: Quantitative characterization of histone post-translational modifications using a stable isotope dimethyl-labeling strategy
作者: Han, Yanpu1;  Lu, Congcong1;  Zhang, Kai1, 2, 3;  Tian, Shanshan2, 3;  Fan, Enguo4;  Chen, Langxing1;  He, Xiwen1;  Zhang, Yukui1, 5
通讯作者: 张锴
刊名: ANALYTICAL METHODS
发表日期: 2015
DOI: 10.1039/c5ay00120j
卷: 7, 期:9, 页:3779-3785
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences ;  Life Sciences & Biomedicine ;  Technology
类目[WOS]: Chemistry, Analytical ;  Food Science & Technology ;  Spectroscopy
研究领域[WOS]: Chemistry ;  Food Science & Technology ;  Spectroscopy
英文摘要: Histone post-translational modifications (PTMs) have been considered to be a major group of important epigenetic marks and play critical roles in the regulation of chromatin-templated biological processes. To date, novel strategies for the quantification of histone PTMs are still highly desirable. Herein, we present an efficient approach to quantitatively characterize histone PTMs using stable isotope dimethyl labeling coupled with mass spectrometry. At first, all of the 3-aminogroups of free lysines are derivatized by heavy formaldehyde to enable an easy distinction of free lysines from those of naturally occurring lysine-dimethylation upon MS analysis. After tryptic digestion, a second derivatization was applied with heavy- and light-stable isotope dimethyl labeling to label the N-termini of tryptic peptides from different sample sources. The mixture was further identified and quantified by HPLC-MS/MS. This method enables the comparison of histone PTMs from multiple sample sources and the quantification of different PTMs at certain amino-acid residues of histones in one single experiment. Thus, it is highly attractive for the identification of epigenetic histone marks.
关键词[WOS]: METHYLATION ;  PROTEOMICS ;  LANGUAGE ;  MARKS
语种: 英语
WOS记录号: WOS:000354042300020
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/143692
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Tianjin Med Univ, Tianjin Key Lab Med Epigenet, Tianjin 300070, Peoples R China
3.Tianjin Med Univ, Sch Basic Med Sci, Tianjin 300070, Peoples R China
4.Univ Freiburg, Inst Biochem & Mol Biol, ZBMZ, D-79104 Freiburg, Germany
5.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Han, Yanpu,Lu, Congcong,Zhang, Kai,et al. Quantitative characterization of histone post-translational modifications using a stable isotope dimethyl-labeling strategy[J]. ANALYTICAL METHODS,2015,7(9):3779-3785.
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