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学科主题物理化学
Epitope imprinted polymer nanoparticles containing fluorescent quantum dots for specific recognition of human serum albumin
Wang, Yi-Zhi1,2; Li, Dong-Yan1,2; He, Xi-Wen1,2; Li, Wen-You1,2; Zhang, Yu-Kui1,2,3; Li WY(李文友); Zhang YK(张玉奎)
关键词Epitope Imprinting Human Serum Albumin Quantum Dots Turn-on Fluorescence Fluorometry
刊名MICROCHIMICA ACTA
2015-06-01
DOI10.1007/s00604-015-1464-1
182期:7-8页:1465-1472
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]QUARTZ-CRYSTAL MICROBALANCE ; DOPED ZNS QDS ; TARGET PROTEIN ; PROTEOMIC ANALYSIS ; SURFACE ; ACID ; NANOCRYSTALS ; QUANTIFICATION ; RESOLUTION ; BIOMARKERS
英文摘要Epitope imprinted polymer nanoparticles (EI-NPs) were prepared by one-pot polymerization of N-isopropylacrylamide in the presence of CdTe quantum dots and an epitope (consisting of amino acids 598 to 609) of human serum albumin (HSA). The resulting EI-NPs exhibit specific recognition ability and enable direct fluorescence quantification of HSA based on a fluorescence turn-on mode. The polymer was characterized by FT-IR, X-ray photoelectron spectroscopy, transmission electron microscopy and dynamic light scattering. The linear calibration graph was obtained in the range of 0.25-5 mu mol center dot mL(-1) with the detection limit of 44.3 nmol center dot mL(-1). The EI-NPs were successfully applied to the direct fluorometric quantification of HSA in samples of human serum. Overall, this approach provides a promising tool to design functional fluorescent materials with protein recognition capability and specific applications in proteomics.
语种英语
WOS记录号WOS:000354414300029
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/143695
专题中国科学院大连化学物理研究所
通讯作者Li WY(李文友); Zhang YK(张玉奎)
作者单位1.Nankai Univ, State Key Lab Med Chem Biol, Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China
2.Nankai Univ, Res Ctr Analyt Sci, Coll Chem, Tianjin 300071, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
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GB/T 7714
Wang, Yi-Zhi,Li, Dong-Yan,He, Xi-Wen,et al. Epitope imprinted polymer nanoparticles containing fluorescent quantum dots for specific recognition of human serum albumin[J]. MICROCHIMICA ACTA,2015,182(7-8):1465-1472.
APA Wang, Yi-Zhi.,Li, Dong-Yan.,He, Xi-Wen.,Li, Wen-You.,Zhang, Yu-Kui.,...&张玉奎.(2015).Epitope imprinted polymer nanoparticles containing fluorescent quantum dots for specific recognition of human serum albumin.MICROCHIMICA ACTA,182(7-8),1465-1472.
MLA Wang, Yi-Zhi,et al."Epitope imprinted polymer nanoparticles containing fluorescent quantum dots for specific recognition of human serum albumin".MICROCHIMICA ACTA 182.7-8(2015):1465-1472.
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