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学科主题: 物理化学
题名: Epitope imprinted polymer nanoparticles containing fluorescent quantum dots for specific recognition of human serum albumin
作者: Wang, Yi-Zhi1, 2;  Li, Dong-Yan1, 2;  He, Xi-Wen1, 2;  Li, Wen-You1, 2;  Zhang, Yu-Kui1, 2, 3
通讯作者: 李文友 ;  张玉奎
关键词: Epitope imprinting ;  Human serum albumin ;  Quantum dots ;  Turn-on fluorescence ;  Fluorometry
刊名: MICROCHIMICA ACTA
发表日期: 2015-06-01
DOI: 10.1007/s00604-015-1464-1
卷: 182, 期:7-8, 页:1465-1472
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Epitope imprinted polymer nanoparticles (EI-NPs) were prepared by one-pot polymerization of N-isopropylacrylamide in the presence of CdTe quantum dots and an epitope (consisting of amino acids 598 to 609) of human serum albumin (HSA). The resulting EI-NPs exhibit specific recognition ability and enable direct fluorescence quantification of HSA based on a fluorescence turn-on mode. The polymer was characterized by FT-IR, X-ray photoelectron spectroscopy, transmission electron microscopy and dynamic light scattering. The linear calibration graph was obtained in the range of 0.25-5 mu mol center dot mL(-1) with the detection limit of 44.3 nmol center dot mL(-1). The EI-NPs were successfully applied to the direct fluorometric quantification of HSA in samples of human serum. Overall, this approach provides a promising tool to design functional fluorescent materials with protein recognition capability and specific applications in proteomics.
关键词[WOS]: QUARTZ-CRYSTAL MICROBALANCE ;  DOPED ZNS QDS ;  TARGET PROTEIN ;  PROTEOMIC ANALYSIS ;  SURFACE ;  ACID ;  NANOCRYSTALS ;  QUANTIFICATION ;  RESOLUTION ;  BIOMARKERS
语种: 英语
WOS记录号: WOS:000354414300029
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/143695
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, State Key Lab Med Chem Biol, Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China
2.Nankai Univ, Res Ctr Analyt Sci, Coll Chem, Tianjin 300071, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Wang, Yi-Zhi,Li, Dong-Yan,He, Xi-Wen,et al. Epitope imprinted polymer nanoparticles containing fluorescent quantum dots for specific recognition of human serum albumin[J]. MICROCHIMICA ACTA,2015,182(7-8):1465-1472.
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