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学科主题: 物理化学
题名: Turn-on Fluorescent Sensing of Glutathione S-Transferase at near-Infrared Region Based on FRET between Gold Nanoclusters and Gold Nanorods
作者: Qin, Long1, 2;  He, Xiwen1, 2;  Chen, Langxing1, 2;  Zhang, Yukui1, 2, 3
通讯作者: 陈朗星
关键词: fluorescence resonance energy transfer ;  gold nanoclusters ;  gold nanorods ;  glutathione S-transferase ;  near-infrared
刊名: ACS APPLIED MATERIALS & INTERFACES
发表日期: 2015-03-18
DOI: 10.1021/acsami.5b00269
卷: 7, 期:10, 页:5965-5971
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Nanoscience & Nanotechnology ;  Materials Science, Multidisciplinary
研究领域[WOS]: Science & Technology - Other Topics ;  Materials Science
英文摘要: A fluorescence resonance energy transfer (FRET) method based on gold nanoclusters capped glutathione (AuNCs@GSH) and amine-terminated gold nanorods (AuNRs) is designed for turn-on and near-infrared region (NIR) sensing of glutathione S-transferase (GST). The absorption band of AuNRs is tuned carefully to maximize the spectra overlap and enhance the efficiency of FRET. The FRET from multiple AuNCs to single AuNR quenches about 70% fluorescence emission of AuNCs. After GST is added, the strong specific interaction of GSH-GST can replace the AuNCs@GSH from AuNRs, FRET based on electrostatic interaction between AuNCs@GSH and AuNRs is switched off. Thus, emission enhancement of AuNCs@GSH is observed. The fluorescent enhancement is linearly with the increasing GST concentration over the range of 2-100 nM GST and the limit of detection for GST is about 1.5 nM.
关键词[WOS]: RESONANCE ENERGY-TRANSFER ;  SEMICONDUCTOR QUANTUM DOTS ;  NANOPARTICLES ;  SURFACE ;  PROBES ;  ELISA ;  QUANTITATION ;  BIOMOLECULES ;  ASSEMBLIES ;  EXPRESSION
语种: 英语
WOS记录号: WOS:000351420300041
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/143703
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin Key Lab Biosensing & Mol Recognit, Res Ctr Analyt Sci,Coll Chem, Tianjin 300071, Peoples R China
2.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300072, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China

Recommended Citation:
Qin, Long,He, Xiwen,Chen, Langxing,et al. Turn-on Fluorescent Sensing of Glutathione S-Transferase at near-Infrared Region Based on FRET between Gold Nanoclusters and Gold Nanorods[J]. ACS APPLIED MATERIALS & INTERFACES,2015,7(10):5965-5971.
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