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学科主题: 物理化学
题名: Teicoplanin bonded sub-2 mu m superficially porous particles for enantioseparation of native amino acids
作者: Min, Yi1, 2;  Sui, Zhigang1;  Liang, Zhen1;  Zhang, Lihua1;  Zhang, Yukui1
通讯作者: 张丽华
关键词: Chiral separation ;  Sub-2 mu m superficially porous particles ;  Native amino acids ;  Teicoplanin
刊名: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
发表日期: 2015-10-10
DOI: 10.1016/j.jpba.2015.05.035
卷: 114, 期:1, 页:247-253
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences ;  Life Sciences & Biomedicine
类目[WOS]: Chemistry, Analytical ;  Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ;  Pharmacology & Pharmacy
英文摘要: Superficially porous particles (SPPs) demonstrate superior efficiency than totally porous particles in chiral separations. In order to obtain high efficiency and fast separation, sub-2 mu m SPPs with high surface area are synthesized, and with teicoplanin bonded, such materials are successfully applied into the rapid enantioseparation of native amino acids. In brief, 1.27 +/- 0.06 mu m nonporous silica particles are prepared by a modified seeded growth method, followed by mesoporous shell fabrication via one-pot templated dissolution and redeposition strategy, and pore size expansion via acid-refluxing. The diameter of the formed SPPs is 1.49 +/- 0.04 mu m, with the shell thickness as 206 nm. Nitrogen physisorption experiments show that the Brunauer-Emmett-Teller (BET) specific surface area is 213.6 m(2)/g and pore size is 9 nm. After teicoplanin derivatization with bonding capacity as 83.3 mu mol/g, the prepared chiral stationary phase is packed into a stainless steel tube with the geometry of 50 mm x 2.1 mm i.d.. In less than 6.4 min, six native amino acids (norleucine, alanine, valine, methionine, leucine, norvaline) are enantioseparated with resolution factors ranging from 1.9 to 5.0. Besides, the resolution for chiral separation is improved with ethanol-water instead of methanol-water as the mobile phase. Moreover, the low temperature gives higher resolution, but longer retention time and higher backpressure. Finally, the effect of flow rate on enantiomeric separation is studied and fast chiral separation within I min is obtained with flow rate of 0.4 mL/min. All these results show that the synthesized teicoplanin bonded sub-2 mu m SPPs have great potential to achieve the enantioseparation of native amino acids with high resolution and rapid speed. (C) 2015 Published by Elsevier B.V.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  CHIRAL STATIONARY PHASES ;  MESOPOROUS SILICA PARTICLES ;  CORE-SHELL ;  COLUMN PERFORMANCE ;  SEPARATION ;  ENANTIOMERS ;  SELECTORS ;  SIZE
语种: 英语
WOS记录号: WOS:000360780700035
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/143708
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Min, Yi,Sui, Zhigang,Liang, Zhen,et al. Teicoplanin bonded sub-2 mu m superficially porous particles for enantioseparation of native amino acids[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2015,114(1):247-253.
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