DICP OpenIR
Subject Area物理化学
Teicoplanin bonded sub-2 mu m superficially porous particles for enantioseparation of native amino acids
Min, Yi1,2; Sui, Zhigang1; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1; Zhang LH(张丽华)
KeywordChiral Separation Sub-2 Mu m Superficially Porous Particles Native Amino Acids Teicoplanin
Source PublicationJOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
2015-10-10
DOI10.1016/j.jpba.2015.05.035
Volume114Issue:1Pages:247-253
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Life Sciences & Biomedicine
WOS SubjectChemistry, Analytical ; Pharmacology & Pharmacy
WOS Research AreaChemistry ; Pharmacology & Pharmacy
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; CHIRAL STATIONARY PHASES ; MESOPOROUS SILICA PARTICLES ; CORE-SHELL ; COLUMN PERFORMANCE ; SEPARATION ; ENANTIOMERS ; SELECTORS ; SIZE
AbstractSuperficially porous particles (SPPs) demonstrate superior efficiency than totally porous particles in chiral separations. In order to obtain high efficiency and fast separation, sub-2 mu m SPPs with high surface area are synthesized, and with teicoplanin bonded, such materials are successfully applied into the rapid enantioseparation of native amino acids. In brief, 1.27 +/- 0.06 mu m nonporous silica particles are prepared by a modified seeded growth method, followed by mesoporous shell fabrication via one-pot templated dissolution and redeposition strategy, and pore size expansion via acid-refluxing. The diameter of the formed SPPs is 1.49 +/- 0.04 mu m, with the shell thickness as 206 nm. Nitrogen physisorption experiments show that the Brunauer-Emmett-Teller (BET) specific surface area is 213.6 m(2)/g and pore size is 9 nm. After teicoplanin derivatization with bonding capacity as 83.3 mu mol/g, the prepared chiral stationary phase is packed into a stainless steel tube with the geometry of 50 mm x 2.1 mm i.d.. In less than 6.4 min, six native amino acids (norleucine, alanine, valine, methionine, leucine, norvaline) are enantioseparated with resolution factors ranging from 1.9 to 5.0. Besides, the resolution for chiral separation is improved with ethanol-water instead of methanol-water as the mobile phase. Moreover, the low temperature gives higher resolution, but longer retention time and higher backpressure. Finally, the effect of flow rate on enantiomeric separation is studied and fast chiral separation within I min is obtained with flow rate of 0.4 mL/min. All these results show that the synthesized teicoplanin bonded sub-2 mu m SPPs have great potential to achieve the enantioseparation of native amino acids with high resolution and rapid speed. (C) 2015 Published by Elsevier B.V.
Language英语
WOS IDWOS:000360780700035
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/143708
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang LH(张丽华)
Affiliation1.Chinese Acad Sci, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Min, Yi,Sui, Zhigang,Liang, Zhen,et al. Teicoplanin bonded sub-2 mu m superficially porous particles for enantioseparation of native amino acids[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2015,114(1):247-253.
APA Min, Yi,Sui, Zhigang,Liang, Zhen,Zhang, Lihua,Zhang, Yukui,&张丽华.(2015).Teicoplanin bonded sub-2 mu m superficially porous particles for enantioseparation of native amino acids.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,114(1),247-253.
MLA Min, Yi,et al."Teicoplanin bonded sub-2 mu m superficially porous particles for enantioseparation of native amino acids".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 114.1(2015):247-253.
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