DICP OpenIR
Subject Area物理化学
Integrated SDS removal and protein digestion by hollow fiber membrane based device for SDS-assisted proteome analysis
Xia, Simin1,2; Yuan, Huiming1; Chen, Yuanbo1,2; Liang, Zheng1; Zhang, Lihua1; Zhang, Yukui1; Zhang LH(张丽华)
KeywordSds Removal On-line Digestion Hollow Fiber Membrane Interface Immobilized Enzymatic Reactor Proteome Analysis
Source PublicationTALANTA
2015-08-15
DOI10.1016/j.talanta.2015.04.011
Volume141Issue:1Pages:235-238
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordSAMPLE PREPARATION METHOD ; TANDEM MASS-SPECTROMETRY ; LIQUID-CHROMATOGRAPHY ; GEL-ELECTROPHORESIS ; PEPTIDE SEPARATION ; IDENTIFICATION
AbstractIn this work, a novel integrated sample preparation device for SDS-assisted proteome analysis was developed, by which proteins dissolved in 4% (w/v) SDS were first diluted by 50% methanol, and then SDS was online removed by a hollow fiber membrane interface (HFMI) with 50 mM ammonium bicarbonate (pH 8.0) as an exchange buffer, finally digested by an immobilized enzyme reactor (IMER). To evaluate the performance of such an integrated device, bovine serum albumin dissolved in 4% (w/v) SDS as a model sample was analyzed; it could be found that similar to that obtained by direct analysis of BSA digests without SDS (the sequence coverage of 60.3 +/- 1.0%, n=3), with HFMI as an interface for SDS removal, BSA was identified with the sequence coverage of 61.0 +/- 1.0% (n=3). However, without SDS removal by HFMI, BSA could not be digested by the IMER and none peptides could be detected. In addition, such an integrated sample preparation device was also applied for the analysis of SDS extracted proteins from rat brain, compared to those obtained by filter-aided sample preparation (FASP), not only the identified protein group and unique peptide number were increased by 12% and 39% respectively, but also the sample pretreatment time was shortened from 24 h to 4 h. All these results demonstrated that such an integrated sample preparation device would provide an alternative tool for SDS assisted proteome analysis. (C) 2015 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000356987500037
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/143709
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang LH(张丽华)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Xia, Simin,Yuan, Huiming,Chen, Yuanbo,et al. Integrated SDS removal and protein digestion by hollow fiber membrane based device for SDS-assisted proteome analysis[J]. TALANTA,2015,141(1):235-238.
APA Xia, Simin.,Yuan, Huiming.,Chen, Yuanbo.,Liang, Zheng.,Zhang, Lihua.,...&张丽华.(2015).Integrated SDS removal and protein digestion by hollow fiber membrane based device for SDS-assisted proteome analysis.TALANTA,141(1),235-238.
MLA Xia, Simin,et al."Integrated SDS removal and protein digestion by hollow fiber membrane based device for SDS-assisted proteome analysis".TALANTA 141.1(2015):235-238.
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2015hQPBXZXYrJ.PDF(510KB)期刊论文作者接受稿开放获取CC BY-NC-SAApplication Full Text
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