DICP OpenIR
Subject Area物理化学
A paired ions scoring algorithm based on Morpheus for simultaneous identification and quantification of proteome samples prepared by isobaric peptide termini labeling strategies
Zhang, Shen1,2; Wu, Qi1,2; Shan, Yichu1; Sui, Zhigang1; Zhang, Lihua1; Zhang, Yukui1; Zhang LH(张丽华)
KeywordBioinformatics Database Search Algorithm Isobaric Peptide Termini Labeling Morpheus Paired Ions Scoring Algorithm Quantification
Source PublicationPROTEOMICS
2015-06-01
DOI10.1002/pmic.201400262
Volume15Issue:11Pages:1781-1788
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology
WOS Research AreaBiochemistry & Molecular Biology
WOS KeywordTANDEM MASS-SPECTROMETRY ; QUANTITATIVE PROTEOMICS ; EXPRESSION ; DATABASES ; MIXTURES ; MS/MS ; TAGS
AbstractThe isobaric peptide termini labeling (IPTL) method is a promising strategy in quantitative proteomics for its high accuracy, while the increased complexity of MS2 spectra originated from the paired b, y ions has adverse effect on the identification and the coverage of quantification. Here, a paired ions scoring algorithm (PISA) based on Morpheus, a database searching algorithm specifically designed for high-resolution MS2 spectra, was proposed to address this issue. PISA was first tested on two 1:1 mixed IPTL datasets, and increases in peptide to spectrum matchings, distinct peptides and protein groups compared to Morpheus itself and MASCOT were shown. Furthermore, the quantification is simultaneously performed and 100% quantification coverage is achieved by PISA since each of the identified peptide to spectrum matchings has several pairs of fragment ions which could be used for quantification. Then the PISA was applied to the relative quantification of human hepatocellular carcinoma cell lines with high and low metastatic potentials prepared by an IPTL strategy.
Language英语
WOS IDWOS:000355727400003
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/143710
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang LH(张丽华)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Shen,Wu, Qi,Shan, Yichu,et al. A paired ions scoring algorithm based on Morpheus for simultaneous identification and quantification of proteome samples prepared by isobaric peptide termini labeling strategies[J]. PROTEOMICS,2015,15(11):1781-1788.
APA Zhang, Shen.,Wu, Qi.,Shan, Yichu.,Sui, Zhigang.,Zhang, Lihua.,...&张丽华.(2015).A paired ions scoring algorithm based on Morpheus for simultaneous identification and quantification of proteome samples prepared by isobaric peptide termini labeling strategies.PROTEOMICS,15(11),1781-1788.
MLA Zhang, Shen,et al."A paired ions scoring algorithm based on Morpheus for simultaneous identification and quantification of proteome samples prepared by isobaric peptide termini labeling strategies".PROTEOMICS 15.11(2015):1781-1788.
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