DICP OpenIR
学科主题物理化学
A paired ions scoring algorithm based on Morpheus for simultaneous identification and quantification of proteome samples prepared by isobaric peptide termini labeling strategies
Zhang, Shen1,2; Wu, Qi1,2; Shan, Yichu1; Sui, Zhigang1; Zhang, Lihua1; Zhang, Yukui1; Zhang LH(张丽华)
关键词Bioinformatics Database Search Algorithm Isobaric Peptide Termini Labeling Morpheus Paired Ions Scoring Algorithm Quantification
刊名PROTEOMICS
2015-06-01
DOI10.1002/pmic.201400262
15期:11页:1781-1788
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]TANDEM MASS-SPECTROMETRY ; QUANTITATIVE PROTEOMICS ; EXPRESSION ; DATABASES ; MIXTURES ; MS/MS ; TAGS
英文摘要The isobaric peptide termini labeling (IPTL) method is a promising strategy in quantitative proteomics for its high accuracy, while the increased complexity of MS2 spectra originated from the paired b, y ions has adverse effect on the identification and the coverage of quantification. Here, a paired ions scoring algorithm (PISA) based on Morpheus, a database searching algorithm specifically designed for high-resolution MS2 spectra, was proposed to address this issue. PISA was first tested on two 1:1 mixed IPTL datasets, and increases in peptide to spectrum matchings, distinct peptides and protein groups compared to Morpheus itself and MASCOT were shown. Furthermore, the quantification is simultaneously performed and 100% quantification coverage is achieved by PISA since each of the identified peptide to spectrum matchings has several pairs of fragment ions which could be used for quantification. Then the PISA was applied to the relative quantification of human hepatocellular carcinoma cell lines with high and low metastatic potentials prepared by an IPTL strategy.
语种英语
WOS记录号WOS:000355727400003
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/143710
专题中国科学院大连化学物理研究所
通讯作者Zhang LH(张丽华)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Shen,Wu, Qi,Shan, Yichu,et al. A paired ions scoring algorithm based on Morpheus for simultaneous identification and quantification of proteome samples prepared by isobaric peptide termini labeling strategies[J]. PROTEOMICS,2015,15(11):1781-1788.
APA Zhang, Shen.,Wu, Qi.,Shan, Yichu.,Sui, Zhigang.,Zhang, Lihua.,...&张丽华.(2015).A paired ions scoring algorithm based on Morpheus for simultaneous identification and quantification of proteome samples prepared by isobaric peptide termini labeling strategies.PROTEOMICS,15(11),1781-1788.
MLA Zhang, Shen,et al."A paired ions scoring algorithm based on Morpheus for simultaneous identification and quantification of proteome samples prepared by isobaric peptide termini labeling strategies".PROTEOMICS 15.11(2015):1781-1788.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
2015u8zAQQ0Pof.PDF(628KB)期刊论文作者接受稿开放获取CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhang, Shen]的文章
[Wu, Qi]的文章
[Shan, Yichu]的文章
百度学术
百度学术中相似的文章
[Zhang, Shen]的文章
[Wu, Qi]的文章
[Shan, Yichu]的文章
必应学术
必应学术中相似的文章
[Zhang, Shen]的文章
[Wu, Qi]的文章
[Shan, Yichu]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。