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学科主题: 物理化学
题名: A paired ions scoring algorithm based on Morpheus for simultaneous identification and quantification of proteome samples prepared by isobaric peptide termini labeling strategies
作者: Zhang, Shen1, 2;  Wu, Qi1, 2;  Shan, Yichu1;  Sui, Zhigang1;  Zhang, Lihua1;  Zhang, Yukui1
通讯作者: 张丽华
关键词: Bioinformatics ;  Database search algorithm ;  Isobaric peptide termini labeling ;  Morpheus ;  Paired ions scoring algorithm ;  Quantification
刊名: PROTEOMICS
发表日期: 2015-06-01
DOI: 10.1002/pmic.201400262
卷: 15, 期:11, 页:1781-1788
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods ;  Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: The isobaric peptide termini labeling (IPTL) method is a promising strategy in quantitative proteomics for its high accuracy, while the increased complexity of MS2 spectra originated from the paired b, y ions has adverse effect on the identification and the coverage of quantification. Here, a paired ions scoring algorithm (PISA) based on Morpheus, a database searching algorithm specifically designed for high-resolution MS2 spectra, was proposed to address this issue. PISA was first tested on two 1:1 mixed IPTL datasets, and increases in peptide to spectrum matchings, distinct peptides and protein groups compared to Morpheus itself and MASCOT were shown. Furthermore, the quantification is simultaneously performed and 100% quantification coverage is achieved by PISA since each of the identified peptide to spectrum matchings has several pairs of fragment ions which could be used for quantification. Then the PISA was applied to the relative quantification of human hepatocellular carcinoma cell lines with high and low metastatic potentials prepared by an IPTL strategy.
关键词[WOS]: TANDEM MASS-SPECTROMETRY ;  QUANTITATIVE PROTEOMICS ;  EXPRESSION ;  DATABASES ;  MIXTURES ;  MS/MS ;  TAGS
语种: 英语
WOS记录号: WOS:000355727400003
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/143710
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China

Recommended Citation:
Zhang, Shen,Wu, Qi,Shan, Yichu,et al. A paired ions scoring algorithm based on Morpheus for simultaneous identification and quantification of proteome samples prepared by isobaric peptide termini labeling strategies[J]. PROTEOMICS,2015,15(11):1781-1788.
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