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学科主题: 物理化学
题名: Partially isobaric peptide termini labeling assisted proteome quantitation based on MS and MS/MS signals
作者: Zhang, Shen1, 2;  Wu, Qi1, 2;  Shan, Yichu1;  Zhou, Yuan1;  Zhang, Lihua1;  Zhang, Yukui1
通讯作者: 张丽华
关键词: Quantitative proteomics ;  Partially isobaric peptide termini ;  labeling ;  MS spectra ;  MS/MS spectra ;  Hca-F ;  Hca-P
刊名: JOURNAL OF PROTEOMICS
发表日期: 2015-01-30
DOI: 10.1016/j.jprot.2014.11.014
卷: 114, 期:1, 页:152-160
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Isotopic labeling and isobaric labeling are two kinds of the typical quantification method that have been widely used in proteomics analysis. Herein, a novel quantitative strategy, partially isobaric peptide termini labeling (PITL), was developed to overcome the drawbacks of each above-mentioned labeling strategy, by simultaneously collecting the quantitative information from both MS and MS/MS spectrum. PITL is based on the site-selective N-terminus dimethylation labeling of peptide alpha-N-termini and the free epsilon-amino group of lysines, resulting in the partially isobaric labeling of peptides. The relative quantification can then be achieved by comparing the intensities of precursor ions in MS spectra and a, b and y ions in MS/MS spectra. The quantitative analysis of differently labeled yeast digests pooled with various ratios indicated the good quantitative accuracy, reproducibility, coverage and wide dynamic range of PITL strategy. Furthermore, PITL was applied to the quantitative proteome analysis of two mouse hepatocarcinoma ascites syngeneic cell lines with low and high lymph node metastasis rates (Hca-F and Hca-P). Given its low cost, simple operation and good accuracy, PITL might have great potential in the quantitative proteome analysis of biological samples.
关键词[WOS]: MASS-SPECTROMETRY ;  QUANTIFICATION ;  SEPARATION ;  SYSTEM ;  TAGS
语种: 英语
WOS记录号: WOS:000348949100013
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/143712
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R & A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China

Recommended Citation:
Zhang, Shen,Wu, Qi,Shan, Yichu,et al. Partially isobaric peptide termini labeling assisted proteome quantitation based on MS and MS/MS signals[J]. JOURNAL OF PROTEOMICS,2015,114(1):152-160.
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