DICP OpenIR
Subject Area物理化学
Partially isobaric peptide termini labeling assisted proteome quantitation based on MS and MS/MS signals
Zhang, Shen1,2; Wu, Qi1,2; Shan, Yichu1; Zhou, Yuan1; Zhang, Lihua1; Zhang, Yukui1; Zhang LH(张丽华)
KeywordQuantitative Proteomics Partially Isobaric Peptide Termini Labeling Ms Spectra Ms/ms Spectra Hca-f Hca-p
Source PublicationJOURNAL OF PROTEOMICS
2015-01-30
DOI10.1016/j.jprot.2014.11.014
Volume114Issue:1Pages:152-160
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemical Research Methods
WOS Research AreaBiochemistry & Molecular Biology
WOS KeywordMASS-SPECTROMETRY ; QUANTIFICATION ; SEPARATION ; SYSTEM ; TAGS
AbstractIsotopic labeling and isobaric labeling are two kinds of the typical quantification method that have been widely used in proteomics analysis. Herein, a novel quantitative strategy, partially isobaric peptide termini labeling (PITL), was developed to overcome the drawbacks of each above-mentioned labeling strategy, by simultaneously collecting the quantitative information from both MS and MS/MS spectrum. PITL is based on the site-selective N-terminus dimethylation labeling of peptide alpha-N-termini and the free epsilon-amino group of lysines, resulting in the partially isobaric labeling of peptides. The relative quantification can then be achieved by comparing the intensities of precursor ions in MS spectra and a, b and y ions in MS/MS spectra. The quantitative analysis of differently labeled yeast digests pooled with various ratios indicated the good quantitative accuracy, reproducibility, coverage and wide dynamic range of PITL strategy. Furthermore, PITL was applied to the quantitative proteome analysis of two mouse hepatocarcinoma ascites syngeneic cell lines with low and high lymph node metastasis rates (Hca-F and Hca-P). Given its low cost, simple operation and good accuracy, PITL might have great potential in the quantitative proteome analysis of biological samples.
Language英语
WOS IDWOS:000348949100013
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/143712
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang LH(张丽华)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R & A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Shen,Wu, Qi,Shan, Yichu,et al. Partially isobaric peptide termini labeling assisted proteome quantitation based on MS and MS/MS signals[J]. JOURNAL OF PROTEOMICS,2015,114(1):152-160.
APA Zhang, Shen.,Wu, Qi.,Shan, Yichu.,Zhou, Yuan.,Zhang, Lihua.,...&张丽华.(2015).Partially isobaric peptide termini labeling assisted proteome quantitation based on MS and MS/MS signals.JOURNAL OF PROTEOMICS,114(1),152-160.
MLA Zhang, Shen,et al."Partially isobaric peptide termini labeling assisted proteome quantitation based on MS and MS/MS signals".JOURNAL OF PROTEOMICS 114.1(2015):152-160.
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