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学科主题: 物理化学
题名: Surface-Imprinted Nanoparticles Prepared with a His-Tag-Anchored Epitope as the Template
作者: Li, Senwu1, 2;  Yang, Kaiguang1;  Liu, Jianxi1, 2;  Jiang, Bo1;  Zhang, Lihua1;  Zhang, Yukui1
通讯作者: 张丽华
刊名: ANALYTICAL CHEMISTRY
发表日期: 2015-05-05
DOI: 10.1021/ac5047246
卷: 87, 期:9, 页:4617-4620
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: The specific recognition of biomolecules by artificial antibodies has inspired fascination among chemists and biologists. Herein, we propose a new method to prepare epitope-oriented surface-imprinted nanoparticles with high template utilization efficiency. Using a His-tag as the anchor to facilitate the epitope immobilization/removal and the self-polymerization of dopamine to control the imprinted shell thickness, the prepared epitope-imprinted nanoparticles show specific recognition of the target protein. Moreover, with improved hydrophilicity of the His-tag-anchored epitope, this method opens up a universal route for imprinting epitopes with various polarities.
关键词[WOS]: SILICA NANOPARTICLES ;  PROTEIN RECOGNITION ;  POLYMERS ;  POLYMERIZATION ;  EXTRACTION ;  SEPARATION ;  CAVITIES
语种: 英语
WOS记录号: WOS:000354153600007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/143715
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Li, Senwu,Yang, Kaiguang,Liu, Jianxi,et al. Surface-Imprinted Nanoparticles Prepared with a His-Tag-Anchored Epitope as the Template[J]. ANALYTICAL CHEMISTRY,2015,87(9):4617-4620.
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