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学科主题: 物理化学
题名: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation
作者: Weng, Yejing1, 2;  Sui, Zhigang1;  Jiang, Hao1, 2;  Shan, Yichu1;  Chen, Lingfan1, 2;  Zhang, Shen1, 2;  Zhang, Lihua1;  Zhang, Yukui1
通讯作者: 张丽华
刊名: Scientific Reports
发表日期: 2015-04-22
DOI: 10.1038/srep09770
卷: 5, 期:1, 页:1
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
英文摘要: Duo to the important roles of N-glycoproteins in various biological processes, the global N-glycoproteome analysis has been paid much attention. However, by current strategies for N-glycoproteome profiling, peptides with glycosylated Asn at N-terminus (PGANs), generated by protease digestion, could hardly be identified, due to the poor deglycosylation capacity by enzymes. However, theoretically, PGANs occupy 10% of N-glycopeptides in the typical tryptic digests. Therefore, in this study, we developed a novel strategy to identify PGANs by releasing N-glycans through the N-terminal site-selective succinylation assisted enzymatic deglycosylation. The obtained PGANs information is beneficial to not only achieve the deep coverage analysis of glycoproteomes, but also discover the new biological functions of such modification.
关键词[WOS]: HYDROPHILIC INTERACTION CHROMATOGRAPHY ;  GLYCOSYLATION SITES ;  PNGASE-F ;  GLYCOPROTEINS ;  ENRICHMENT ;  OLIGOSACCHARIDES ;  GLYCOPEPTIDES ;  HETEROGENEITY ;  PROTEOMICS ;  IDENTIFY
语种: 英语
WOS记录号: WOS:000353283500001
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/143716
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Weng, Yejing,Sui, Zhigang,Jiang, Hao,et al. Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation[J]. Scientific Reports,2015,5(1):1.
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