DICP OpenIR
Subject Area物理化学
Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation
Weng, Yejing1,2; Sui, Zhigang1; Jiang, Hao1,2; Shan, Yichu1; Chen, Lingfan1,2; Zhang, Shen1,2; Zhang, Lihua1; Zhang, Yukui1; Zhang LH(张丽华)
Source PublicationScientific Reports
2015-04-22
DOI10.1038/srep09770
Volume5Issue:1Pages:1
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology
WOS SubjectMultidisciplinary Sciences
WOS Research AreaScience & Technology - Other Topics
WOS KeywordHYDROPHILIC INTERACTION CHROMATOGRAPHY ; GLYCOSYLATION SITES ; PNGASE-F ; GLYCOPROTEINS ; ENRICHMENT ; OLIGOSACCHARIDES ; GLYCOPEPTIDES ; HETEROGENEITY ; PROTEOMICS ; IDENTIFY
AbstractDuo to the important roles of N-glycoproteins in various biological processes, the global N-glycoproteome analysis has been paid much attention. However, by current strategies for N-glycoproteome profiling, peptides with glycosylated Asn at N-terminus (PGANs), generated by protease digestion, could hardly be identified, due to the poor deglycosylation capacity by enzymes. However, theoretically, PGANs occupy 10% of N-glycopeptides in the typical tryptic digests. Therefore, in this study, we developed a novel strategy to identify PGANs by releasing N-glycans through the N-terminal site-selective succinylation assisted enzymatic deglycosylation. The obtained PGANs information is beneficial to not only achieve the deep coverage analysis of glycoproteomes, but also discover the new biological functions of such modification.
Language英语
WOS IDWOS:000353283500001
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/143716
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang LH(张丽华)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Weng, Yejing,Sui, Zhigang,Jiang, Hao,et al. Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation[J]. Scientific Reports,2015,5(1):1.
APA Weng, Yejing.,Sui, Zhigang.,Jiang, Hao.,Shan, Yichu.,Chen, Lingfan.,...&张丽华.(2015).Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation.Scientific Reports,5(1),1.
MLA Weng, Yejing,et al."Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation".Scientific Reports 5.1(2015):1.
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