DICP OpenIR
Subject Area物理化学
Polyethyleneimine-modified graphene oxide nanocomposites for effective protein functionalization
Weng, Yejing1,2; Jiang, Bo1; Yang, Kaiguang1; Sui, Zhigang1; Zhang, Lihua1; Zhang, Yukui1; Zhang LH(张丽华)
Source PublicationNANOSCALE
2015
DOI10.1039/c5nr03370e
Volume7Issue:34Pages:14284-14291
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Technology
WOS SubjectChemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
WOS Research AreaChemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS KeywordCONCANAVALIN-A ; ENZYME IMMOBILIZATION ; SELECTIVE ENRICHMENT ; GLYCOPROTEINS ; MATRIX ; NANOPARTICLES ; GLYCOSYLATION ; NANOSHEETS ; REDUCTION ; GRAPHITE
AbstractA facile method to prepare a biocompatible graphene oxide (GO)-based substrate for protein immobilization was developed to overcome the drawbacks of GO, such as the strong electrostatic and hydrophobic interactions which could potentially alter the conformation and biological activity of proteins. The GO was coated with hydrophilic branched polyethyleneimine (BPEI), while Concanavalin A (Con A) as a model lectin protein was employed to fabricate the functionalized composites to evaluate the feasibility of this strategy. The composites exhibit an extremely high binding capacity for glycoproteins (i.e. IgG 538.3 mg g(-1)), which are superior to other immobilized materials. Moreover, they can work well in 500-fold non-glycoprotein interference and even in complex biological samples. All these data suggest that the GO@BPEI composites will have great potential as scaffolds for proteins fully exerting their biofunctions.
Language英语
WOS IDWOS:000360117200012
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/143725
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang LH(张丽华)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Weng, Yejing,Jiang, Bo,Yang, Kaiguang,et al. Polyethyleneimine-modified graphene oxide nanocomposites for effective protein functionalization[J]. NANOSCALE,2015,7(34):14284-14291.
APA Weng, Yejing.,Jiang, Bo.,Yang, Kaiguang.,Sui, Zhigang.,Zhang, Lihua.,...&张丽华.(2015).Polyethyleneimine-modified graphene oxide nanocomposites for effective protein functionalization.NANOSCALE,7(34),14284-14291.
MLA Weng, Yejing,et al."Polyethyleneimine-modified graphene oxide nanocomposites for effective protein functionalization".NANOSCALE 7.34(2015):14284-14291.
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