DICP OpenIR
Subject Area物理化学
A peptide N-terminal protection strategy for comprehensive glycoproteome analysis using hydrazide chemistry based method
Huang, Junfeng1,2; Qin, Hongqiang1; Sun, Zhen1,2; Huang, Guang1,2; Mao, Jiawei1,2; Cheng, Kai1,2; Zhang, Zhang1,2; Wan, Hao1,3; Yao, Yating1,2; Dong, Jing1; Zhu, Jun1,2; Wang, Fangjun1; Ye, Mingliang1; Zou, Hanfa1; Ye ML(叶明亮); Zou HF(邹汉法)
Source PublicationSCIENTIFIC REPORTS
2015-05-11
DOI10.1038/srep10164
Volume5Issue:1Pages:10164
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology
WOS SubjectMultidisciplinary Sciences
WOS Research AreaScience & Technology - Other Topics
WOS KeywordHYDROPHILIC INTERACTION CHROMATOGRAPHY ; TITANIUM-DIOXIDE CHROMATOGRAPHY ; SOLID-PHASE EXTRACTION ; MASS-SPECTROMETRY ; PHOSPHOPROTEOME ANALYSIS ; QUANTITATIVE PROTEOMICS ; PROTEIN GLYCOSYLATION ; LINKED GLYCOPROTEINS ; SELECTIVE ENRICHMENT ; PERIODATE-OXIDATION
AbstractEnrichment of glycopeptides by hydrazide chemistry (HC) is a popular method for glycoproteomics analysis. However, possible side reactions of peptide backbones during the glycan oxidation in this method have not been comprehensively studied. Here, we developed a proteomics approach to locate such side reactions and found several types of the side reactions that could seriously compromise the performance of glycoproteomics analysis. Particularly, the HC method failed to identify N-terminal Ser/Thr glycopeptides because the oxidation of vicinal amino alcohol on these peptides generates aldehyde groups and after they are covalently coupled to HC beads, these peptides cannot be released by PNGase F for identification. To overcome this drawback, we apply a peptide N-terminal protection strategy in which primary amine groups on peptides are chemically blocked via dimethyl labeling, thus the vicinal amino alcohols on peptide N-termini are eliminated. Our results showed that this strategy successfully prevented the oxidation of peptide N-termini and significantly improved the coverage of glycoproteome.
Language英语
WOS IDWOS:000354275000001
Citation statistics
Cited Times:31[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/143752
Collection中国科学院大连化学物理研究所
Corresponding AuthorYe ML(叶明亮); Zou HF(邹汉法)
Affiliation1.Chinese Acad Sci, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China
Recommended Citation
GB/T 7714
Huang, Junfeng,Qin, Hongqiang,Sun, Zhen,et al. A peptide N-terminal protection strategy for comprehensive glycoproteome analysis using hydrazide chemistry based method[J]. SCIENTIFIC REPORTS,2015,5(1):10164.
APA Huang, Junfeng.,Qin, Hongqiang.,Sun, Zhen.,Huang, Guang.,Mao, Jiawei.,...&邹汉法.(2015).A peptide N-terminal protection strategy for comprehensive glycoproteome analysis using hydrazide chemistry based method.SCIENTIFIC REPORTS,5(1),10164.
MLA Huang, Junfeng,et al."A peptide N-terminal protection strategy for comprehensive glycoproteome analysis using hydrazide chemistry based method".SCIENTIFIC REPORTS 5.1(2015):10164.
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