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学科主题物理化学
Facile Synthesis of Guanidyl-Functionalized Magnetic Polymer Microspheres for Tunable and Specific Capture of Global Phosphopeptides or Only Multiphosphopeptides
Xiong, Zhichao1; Chen, Yajing1; Zhang, Lingyi1; Ren, Jun1; Zhang, Quanqing2; Ye, Mingliang2; Zhang, Weibing1; Zou, Hanfa2; Zhang WB(张维冰); Zou HF(邹汉法)
关键词Magnetic Microspheres Guanidyl Tunable Enrichment Ability Phosphorylated Peptides Mass Spectrum
刊名ACS APPLIED MATERIALS & INTERFACES
2014-12-24
DOI10.1021/am506882b
6期:24页:22743-22750
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Technology
类目[WOS]Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
研究领域[WOS]Science & Technology - Other Topics ; Materials Science
关键词[WOS]HIGHLY SELECTIVE ENRICHMENT ; PHOSPHORYLATED PEPTIDES ; RAPID SEPARATION ; AFFINITY PROBE ; IRON-OXIDE ; NANOPARTICLES ; SHELL ; IMMOBILIZATION ; GLYCOPEPTIDES ; COMPOSITES
英文摘要The highly selective and efficient capture of heterogeneous types of phosphopeptides is critical for comprehensive and in-depth phosphoproteome analysis, but it still remains a challenge since the lack of affinity material with large binding capacity and controllable specificity. Here, a new affinity material was prepared to improve the enrichment capacity and endue the tunable specificity by introducing guanidyl onto poly(glycidyl methacrylate) (PGMA) modified Fe3O4 microsphere (denoted as Fe3O4@PGMA-Guanidyl). The thick polymer shell endows the composite microsphere with large amount of guanidyl and is beneficial to enhancing the affinity interaction between phosphopeptides and the material. Interestingly, the Fe3O4@PGMA-Guanidyl possesses tunable enriching ability for global phosphopeptides or only multiphosphopeptides through simple regulation of buffer composition. The composite has large enrichment capacity (200 mg g(-1)), extremely high detection sensitivity (0.5 fmol), high enrichment recovery (91.30%), great specificity, and rapid magnetic separation. Moreover, the result of the application to capture of phosphopeptides from tryptic digest of nonfat milk has demonstrated the great potential of Fe3O4@PGMA-Guanidyl in detection and identification of low-abundance phosphopeptides of interest in biological sample.
语种英语
WOS记录号WOS:000347139400120
引用统计
被引频次:28[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/143783
专题中国科学院大连化学物理研究所
通讯作者Zhang WB(张维冰); Zou HF(邹汉法)
作者单位1.E China Univ Sci & Technol, Dept Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
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Xiong, Zhichao,Chen, Yajing,Zhang, Lingyi,et al. Facile Synthesis of Guanidyl-Functionalized Magnetic Polymer Microspheres for Tunable and Specific Capture of Global Phosphopeptides or Only Multiphosphopeptides[J]. ACS APPLIED MATERIALS & INTERFACES,2014,6(24):22743-22750.
APA Xiong, Zhichao.,Chen, Yajing.,Zhang, Lingyi.,Ren, Jun.,Zhang, Quanqing.,...&邹汉法.(2014).Facile Synthesis of Guanidyl-Functionalized Magnetic Polymer Microspheres for Tunable and Specific Capture of Global Phosphopeptides or Only Multiphosphopeptides.ACS APPLIED MATERIALS & INTERFACES,6(24),22743-22750.
MLA Xiong, Zhichao,et al."Facile Synthesis of Guanidyl-Functionalized Magnetic Polymer Microspheres for Tunable and Specific Capture of Global Phosphopeptides or Only Multiphosphopeptides".ACS APPLIED MATERIALS & INTERFACES 6.24(2014):22743-22750.
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