中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
学科主题: 物理化学
题名: Facile Synthesis of Guanidyl-Functionalized Magnetic Polymer Microspheres for Tunable and Specific Capture of Global Phosphopeptides or Only Multiphosphopeptides
作者: Xiong, Zhichao1;  Chen, Yajing1;  Zhang, Lingyi1;  Ren, Jun1;  Zhang, Quanqing2;  Ye, Mingliang2;  Zhang, Weibing1;  Zou, Hanfa2
通讯作者: 张维冰 ;  邹汉法
关键词: magnetic microspheres ;  guanidyl ;  tunable enrichment ability ;  phosphorylated peptides ;  mass spectrum
刊名: ACS APPLIED MATERIALS & INTERFACES
发表日期: 2014-12-24
DOI: 10.1021/am506882b
卷: 6, 期:24, 页:22743-22750
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Nanoscience & Nanotechnology ;  Materials Science, Multidisciplinary
研究领域[WOS]: Science & Technology - Other Topics ;  Materials Science
英文摘要: The highly selective and efficient capture of heterogeneous types of phosphopeptides is critical for comprehensive and in-depth phosphoproteome analysis, but it still remains a challenge since the lack of affinity material with large binding capacity and controllable specificity. Here, a new affinity material was prepared to improve the enrichment capacity and endue the tunable specificity by introducing guanidyl onto poly(glycidyl methacrylate) (PGMA) modified Fe3O4 microsphere (denoted as Fe3O4@PGMA-Guanidyl). The thick polymer shell endows the composite microsphere with large amount of guanidyl and is beneficial to enhancing the affinity interaction between phosphopeptides and the material. Interestingly, the Fe3O4@PGMA-Guanidyl possesses tunable enriching ability for global phosphopeptides or only multiphosphopeptides through simple regulation of buffer composition. The composite has large enrichment capacity (200 mg g(-1)), extremely high detection sensitivity (0.5 fmol), high enrichment recovery (91.30%), great specificity, and rapid magnetic separation. Moreover, the result of the application to capture of phosphopeptides from tryptic digest of nonfat milk has demonstrated the great potential of Fe3O4@PGMA-Guanidyl in detection and identification of low-abundance phosphopeptides of interest in biological sample.
关键词[WOS]: HIGHLY SELECTIVE ENRICHMENT ;  PHOSPHORYLATED PEPTIDES ;  RAPID SEPARATION ;  AFFINITY PROBE ;  IRON-OXIDE ;  NANOPARTICLES ;  SHELL ;  IMMOBILIZATION ;  GLYCOPEPTIDES ;  COMPOSITES
语种: 英语
WOS记录号: WOS:000347139400120
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/143783
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
2015DdfSIJ2dEY.PDF(3384KB)期刊论文作者接受稿开放获取View Download

作者单位: 1.E China Univ Sci & Technol, Dept Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Xiong, Zhichao,Chen, Yajing,Zhang, Lingyi,et al. Facile Synthesis of Guanidyl-Functionalized Magnetic Polymer Microspheres for Tunable and Specific Capture of Global Phosphopeptides or Only Multiphosphopeptides[J]. ACS APPLIED MATERIALS & INTERFACES,2014,6(24):22743-22750.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Xiong, Zhichao]'s Articles
 [Chen, Yajing]'s Articles
 [Zhang, Lingyi]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Xiong, Zhichao]‘s Articles
 [Chen, Yajing]‘s Articles
 [Zhang, Lingyi]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
文件名: 2015DdfSIJ2dEY.PDF
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace