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学科主题: 物理化学
题名: 1.9 mu m superficially porous packing material with radially oriented pores and tailored pore size for ultra-fast separation of small molecules and biomolecules
作者: Min, Yi1, 2;  Jiang, Bo1, 2;  Wu, Ci1, 2;  Xia, Simin1, 2;  Zhang, Xiaodan1;  Liang, Zhen1;  Zhang, Lihua1;  Zhang, Yukui1
通讯作者: 张丽华
关键词: Superficially porous silica particles ;  1.9 mu m ;  Radially oriented pores ;  Pore size ;  Peptides ;  Proteins
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2014-08-22
DOI: 10.1016/j.chroma.2014.06.049
卷: 1356, 期:1, 页:148-156
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: In this work, 1.9 mu m reversed-phase packing materials with superficially porous structure were prepared to achieve the rapid and high efficient separation of peptides and proteins. The silica particles were synthesized via three steps, nonporous silica particle preparation by a modified seeded growth method, mesoporous shell formation by a one pot templated dissolution and redeposition strategy, and pore size expansion via acid-refluxing. By such a method, 1.9 mu m superficially porous materials with 0.18 mu m shell thickness and tailored pore diameter (10 nm, 15 nm) were obtained. After pore enlargement, the formerly dense arrays of mesoporous structure changed, the radially oriented pores dominated the superficially porous structure. The chromatographic performance of such particles was investigated after C18 derivatization. For packing materials with 1.9 mu m diameter and 10 nm pore size, the column efficiency could reach 211,300 plates per m for naphthalene. To achieve the high resolution separation of peptides and proteins, particles with pore diameter of 15 nm were tailored, by which the baseline separation of 5 peptides and 5 intact proteins could be respectively achieved within I min, demonstrating the superiority in the high efficiency and high throughput analysis of biomolecules. Furthermore, BSA digests were well separated with peak capacity of 120 in 30 min on a 15 cm-long column. Finally, we compared our columns with a 1.7 mu m Kinetex C18 column under the same conditions, our particles with 10 nm pore size demonstrated similar performance for separation of the large intact proteins. Moreover, the particles with 15 nm pore size showed more symmetrical peaks for the separation of large proteins (BSA, OVA and IgG) and provided rapid separation of protein extracts from Escherichia coli in 5 min. All these results indicated that the synthesized 1.9 mu m superficially porous silica packing materials would be promising in the ultra-fast and high-resolution separation,of biomolecules. (C) 2014 Elsevier B.V. All rights reserved.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  CORE-SHELL PARTICLES ;  SILICA MICROSPHERES ;  COLUMNS ;  MACROMOLECULES ;  EFFICIENCY ;  PROTEINS ;  HPLC
语种: 英语
WOS记录号: WOS:000340302600015
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/143856
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Key Laboratoty Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China

Recommended Citation:
Min, Yi,Jiang, Bo,Wu, Ci,et al. 1.9 mu m superficially porous packing material with radially oriented pores and tailored pore size for ultra-fast separation of small molecules and biomolecules[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1356(1):148-156.
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