DICP OpenIR
Subject Area物理化学
1.9 mu m superficially porous packing material with radially oriented pores and tailored pore size for ultra-fast separation of small molecules and biomolecules
Min, Yi1,2; Jiang, Bo1,2; Wu, Ci1,2; Xia, Simin1,2; Zhang, Xiaodan1; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1; Zhang LH(张丽华)
KeywordSuperficially Porous Silica Particles 1.9 Mu m Radially Oriented Pores Pore Size Peptides Proteins
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2014-08-22
DOI10.1016/j.chroma.2014.06.049
Volume1356Issue:1Pages:148-156
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; CORE-SHELL PARTICLES ; SILICA MICROSPHERES ; COLUMNS ; MACROMOLECULES ; EFFICIENCY ; PROTEINS ; HPLC
AbstractIn this work, 1.9 mu m reversed-phase packing materials with superficially porous structure were prepared to achieve the rapid and high efficient separation of peptides and proteins. The silica particles were synthesized via three steps, nonporous silica particle preparation by a modified seeded growth method, mesoporous shell formation by a one pot templated dissolution and redeposition strategy, and pore size expansion via acid-refluxing. By such a method, 1.9 mu m superficially porous materials with 0.18 mu m shell thickness and tailored pore diameter (10 nm, 15 nm) were obtained. After pore enlargement, the formerly dense arrays of mesoporous structure changed, the radially oriented pores dominated the superficially porous structure. The chromatographic performance of such particles was investigated after C18 derivatization. For packing materials with 1.9 mu m diameter and 10 nm pore size, the column efficiency could reach 211,300 plates per m for naphthalene. To achieve the high resolution separation of peptides and proteins, particles with pore diameter of 15 nm were tailored, by which the baseline separation of 5 peptides and 5 intact proteins could be respectively achieved within I min, demonstrating the superiority in the high efficiency and high throughput analysis of biomolecules. Furthermore, BSA digests were well separated with peak capacity of 120 in 30 min on a 15 cm-long column. Finally, we compared our columns with a 1.7 mu m Kinetex C18 column under the same conditions, our particles with 10 nm pore size demonstrated similar performance for separation of the large intact proteins. Moreover, the particles with 15 nm pore size showed more symmetrical peaks for the separation of large proteins (BSA, OVA and IgG) and provided rapid separation of protein extracts from Escherichia coli in 5 min. All these results indicated that the synthesized 1.9 mu m superficially porous silica packing materials would be promising in the ultra-fast and high-resolution separation,of biomolecules. (C) 2014 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000340302600015
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/143856
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang LH(张丽华)
Affiliation1.Chinese Acad Sci, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Key Laboratoty Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Min, Yi,Jiang, Bo,Wu, Ci,et al. 1.9 mu m superficially porous packing material with radially oriented pores and tailored pore size for ultra-fast separation of small molecules and biomolecules[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1356(1):148-156.
APA Min, Yi.,Jiang, Bo.,Wu, Ci.,Xia, Simin.,Zhang, Xiaodan.,...&张丽华.(2014).1.9 mu m superficially porous packing material with radially oriented pores and tailored pore size for ultra-fast separation of small molecules and biomolecules.JOURNAL OF CHROMATOGRAPHY A,1356(1),148-156.
MLA Min, Yi,et al."1.9 mu m superficially porous packing material with radially oriented pores and tailored pore size for ultra-fast separation of small molecules and biomolecules".JOURNAL OF CHROMATOGRAPHY A 1356.1(2014):148-156.
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