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学科主题: 物理化学
题名: A Microfluidic-Based Multi-Shear Device for Investigating the Effects of Low Fluid-Induced Stresses on Osteoblasts
作者: Yu, Weiliang1, 2;  Qu, Hong2;  Hu, Guoqing4;  Zhang, Qian1;  Song, Kui4;  Guan, Haijie1;  Liu, Tingjiao1;  Qin, Jianhua3
通讯作者: 秦建华 ;  TingjiaoLiu
刊名: PLOS ONE
发表日期: 2014-02-27
DOI: 10.1371/journal.pone.0089966
卷: 9, 期:2, 页:89966
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
英文摘要: Interstitial fluid flow (IFF) within the extracellular matrix (ECM) produces low magnitude shear stresses on cells. Fluid flow-induced stress (FSS) plays an important role during tissue morphogenesis. To investigate the effect of low FSS generated by IFF on cells, we developed a microfluidic-based cell culture device that can generate multiple low shear stresses. By changing the length and width of the flow-in channels, different continuous low level shear stresses could be generated in individual cell culture chambers. Numerical calculations demonstrate uniform shear stress distributions of the major cell culture area of each chamber. This calculation is further confirmed by the wall shear stress curves. The effects of low FSS on MC3T3-E1 proliferation and differentiation were studied using this device. It was found that FSS ranging from 1.5 to 52.6 mu Pa promoted MC3T3-E1 proliferation and differentiation, but FSS over 412 mu Pa inhibited the proliferation and differentiation of MC3T3-E1 cells. FSS ranging from 1.5 to 52.6 mu Pa also increased the expression of Runx2, a key transcription factor regulating osteoblast differentiation. It is suggested that Runx2 might be an important regulator in low FSS-induced MC3T3-E1 differentiation. This device allows for detailed study of the effect of low FSS on the behaviors of cells; thus, it would be a useful tool for analysis of the effects of IFF-induced shear stresses on cells.
关键词[WOS]: MARROW STROMAL CELLS ;  BONE-MARROW ;  IN-VITRO ;  INTERSTITIAL CONVECTION ;  MATRIX PRODUCTION ;  FLOW ;  DIFFERENTIATION ;  INCREASES ;  ENVIRONMENT ;  ACTIVATION
语种: 英语
WOS记录号: WOS:000332390800061
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/143915
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Med Univ, Coll Stomatol, Sect Oral Pathol, Dalian, Peoples R China
2.Dalian Med Univ, Coll Stomatol, Sect Orthodont, Dalian, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian, Peoples R China
4.Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech LNM, Beijing 100080, Peoples R China

Recommended Citation:
Yu, Weiliang,Qu, Hong,Hu, Guoqing,et al. A Microfluidic-Based Multi-Shear Device for Investigating the Effects of Low Fluid-Induced Stresses on Osteoblasts[J]. PLOS ONE,2014,9(2):89966.
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