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学科主题物理化学
A Microfluidic-Based Multi-Shear Device for Investigating the Effects of Low Fluid-Induced Stresses on Osteoblasts
Yu, Weiliang1,2; Qu, Hong2; Hu, Guoqing4; Zhang, Qian1; Song, Kui4; Guan, Haijie1; Liu, Tingjiao1; Qin, Jianhua3; Qin JH(秦建华); TingjiaoLiu
刊名PLOS ONE
2014-02-27
DOI10.1371/journal.pone.0089966
9期:2页:89966
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]MARROW STROMAL CELLS ; BONE-MARROW ; IN-VITRO ; INTERSTITIAL CONVECTION ; MATRIX PRODUCTION ; FLOW ; DIFFERENTIATION ; INCREASES ; ENVIRONMENT ; ACTIVATION
英文摘要Interstitial fluid flow (IFF) within the extracellular matrix (ECM) produces low magnitude shear stresses on cells. Fluid flow-induced stress (FSS) plays an important role during tissue morphogenesis. To investigate the effect of low FSS generated by IFF on cells, we developed a microfluidic-based cell culture device that can generate multiple low shear stresses. By changing the length and width of the flow-in channels, different continuous low level shear stresses could be generated in individual cell culture chambers. Numerical calculations demonstrate uniform shear stress distributions of the major cell culture area of each chamber. This calculation is further confirmed by the wall shear stress curves. The effects of low FSS on MC3T3-E1 proliferation and differentiation were studied using this device. It was found that FSS ranging from 1.5 to 52.6 mu Pa promoted MC3T3-E1 proliferation and differentiation, but FSS over 412 mu Pa inhibited the proliferation and differentiation of MC3T3-E1 cells. FSS ranging from 1.5 to 52.6 mu Pa also increased the expression of Runx2, a key transcription factor regulating osteoblast differentiation. It is suggested that Runx2 might be an important regulator in low FSS-induced MC3T3-E1 differentiation. This device allows for detailed study of the effect of low FSS on the behaviors of cells; thus, it would be a useful tool for analysis of the effects of IFF-induced shear stresses on cells.
语种英语
WOS记录号WOS:000332390800061
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/143915
专题中国科学院大连化学物理研究所
通讯作者Qin JH(秦建华); TingjiaoLiu
作者单位1.Dalian Med Univ, Coll Stomatol, Sect Oral Pathol, Dalian, Peoples R China
2.Dalian Med Univ, Coll Stomatol, Sect Orthodont, Dalian, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian, Peoples R China
4.Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech LNM, Beijing 100080, Peoples R China
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Yu, Weiliang,Qu, Hong,Hu, Guoqing,et al. A Microfluidic-Based Multi-Shear Device for Investigating the Effects of Low Fluid-Induced Stresses on Osteoblasts[J]. PLOS ONE,2014,9(2):89966.
APA Yu, Weiliang.,Qu, Hong.,Hu, Guoqing.,Zhang, Qian.,Song, Kui.,...&TingjiaoLiu.(2014).A Microfluidic-Based Multi-Shear Device for Investigating the Effects of Low Fluid-Induced Stresses on Osteoblasts.PLOS ONE,9(2),89966.
MLA Yu, Weiliang,et al."A Microfluidic-Based Multi-Shear Device for Investigating the Effects of Low Fluid-Induced Stresses on Osteoblasts".PLOS ONE 9.2(2014):89966.
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