DICP OpenIR
Subject Area物理化学
Quantitatively Mapping Cellular Viscosity with Detailed Organelle Information via a Designed PET Fluorescent Probe
Liu, Tianyu1,2; Liu, Xiaogang3; Spring, David R.4; Qian, Xuhong5; Cui, Jingnan2; Xu, Zhaochao1; Xu ZC(徐兆超); Cui JN(崔京南); DavidSpring
Source PublicationSCIENTIFIC REPORTS
2014-06-24
DOI10.1038/srep05418
Volume4Issue:1Pages:05418
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology
WOS SubjectMultidisciplinary Sciences
WOS Research AreaScience & Technology - Other Topics
WOS KeywordPHOTOINDUCED ELECTRON-TRANSFER ; NITRIC-OXIDE SYNTHASE ; MOLECULAR ROTORS ; LIVE CELLS ; CONFORMATIONAL DYNAMICS ; MEMBRANE VISCOSITY ; EXCITED-STATE ; LIVING CELLS ; SENSORS ; DENSITY
AbstractViscosity is a fundamental physical parameter that influences diffusion in biological processes. The distribution of intracellular viscosity is highly heterogeneous, and it is challenging to obtain a full map of cellular viscosity with detailed organelle information. In this work, we report 1 as the first fluorescent viscosity probe which is able to quantitatively map cellular viscosity with detailed organelle information based on the PET mechanism. This probe exhibited a significant ratiometric fluorescence intensity enhancement as solvent viscosity increases. The emission intensity increase was attributed to combined effects of the inhibition of PET due to restricted conformational access (favorable for FRET, but not for PET), and the decreased PET efficiency caused by viscosity-dependent twisted intramolecular charge transfer (TICT). A full map of subcellular viscosity was successfully constructed via fluorescent ratiometric detection and fluorescence lifetime imaging; it was found that lysosomal regions in a cell possess the highest viscosity, followed by mitochondrial regions.
Language英语
WOS IDWOS:000337889600002
Citation statistics
Cited Times:56[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/143917
Collection中国科学院大连化学物理研究所
Corresponding AuthorXu ZC(徐兆超); Cui JN(崔京南); DavidSpring
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China
3.Univ Cambridge, Dept Phys, Cavendish Lab, Cambridge CB2 1TN, England
4.Univ Cambridge, Dept Chem, Cambridge CB2 1TN, England
5.E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China
Recommended Citation
GB/T 7714
Liu, Tianyu,Liu, Xiaogang,Spring, David R.,et al. Quantitatively Mapping Cellular Viscosity with Detailed Organelle Information via a Designed PET Fluorescent Probe[J]. SCIENTIFIC REPORTS,2014,4(1):05418.
APA Liu, Tianyu.,Liu, Xiaogang.,Spring, David R..,Qian, Xuhong.,Cui, Jingnan.,...&DavidSpring.(2014).Quantitatively Mapping Cellular Viscosity with Detailed Organelle Information via a Designed PET Fluorescent Probe.SCIENTIFIC REPORTS,4(1),05418.
MLA Liu, Tianyu,et al."Quantitatively Mapping Cellular Viscosity with Detailed Organelle Information via a Designed PET Fluorescent Probe".SCIENTIFIC REPORTS 4.1(2014):05418.
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