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学科主题物理化学
Quantitatively Mapping Cellular Viscosity with Detailed Organelle Information via a Designed PET Fluorescent Probe
Liu, Tianyu1,2; Liu, Xiaogang3; Spring, David R.4; Qian, Xuhong5; Cui, Jingnan2; Xu, Zhaochao1; Xu ZC(徐兆超); Cui JN(崔京南); DavidSpring
刊名SCIENTIFIC REPORTS
2014-06-24
DOI10.1038/srep05418
4期:1页:05418
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]PHOTOINDUCED ELECTRON-TRANSFER ; NITRIC-OXIDE SYNTHASE ; MOLECULAR ROTORS ; LIVE CELLS ; CONFORMATIONAL DYNAMICS ; MEMBRANE VISCOSITY ; EXCITED-STATE ; LIVING CELLS ; SENSORS ; DENSITY
英文摘要Viscosity is a fundamental physical parameter that influences diffusion in biological processes. The distribution of intracellular viscosity is highly heterogeneous, and it is challenging to obtain a full map of cellular viscosity with detailed organelle information. In this work, we report 1 as the first fluorescent viscosity probe which is able to quantitatively map cellular viscosity with detailed organelle information based on the PET mechanism. This probe exhibited a significant ratiometric fluorescence intensity enhancement as solvent viscosity increases. The emission intensity increase was attributed to combined effects of the inhibition of PET due to restricted conformational access (favorable for FRET, but not for PET), and the decreased PET efficiency caused by viscosity-dependent twisted intramolecular charge transfer (TICT). A full map of subcellular viscosity was successfully constructed via fluorescent ratiometric detection and fluorescence lifetime imaging; it was found that lysosomal regions in a cell possess the highest viscosity, followed by mitochondrial regions.
语种英语
WOS记录号WOS:000337889600002
引用统计
被引频次:43[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/143917
专题中国科学院大连化学物理研究所
通讯作者Xu ZC(徐兆超); Cui JN(崔京南); DavidSpring
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China
3.Univ Cambridge, Dept Phys, Cavendish Lab, Cambridge CB2 1TN, England
4.Univ Cambridge, Dept Chem, Cambridge CB2 1TN, England
5.E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China
推荐引用方式
GB/T 7714
Liu, Tianyu,Liu, Xiaogang,Spring, David R.,et al. Quantitatively Mapping Cellular Viscosity with Detailed Organelle Information via a Designed PET Fluorescent Probe[J]. SCIENTIFIC REPORTS,2014,4(1):05418.
APA Liu, Tianyu.,Liu, Xiaogang.,Spring, David R..,Qian, Xuhong.,Cui, Jingnan.,...&DavidSpring.(2014).Quantitatively Mapping Cellular Viscosity with Detailed Organelle Information via a Designed PET Fluorescent Probe.SCIENTIFIC REPORTS,4(1),05418.
MLA Liu, Tianyu,et al."Quantitatively Mapping Cellular Viscosity with Detailed Organelle Information via a Designed PET Fluorescent Probe".SCIENTIFIC REPORTS 4.1(2014):05418.
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