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学科主题: 物理化学
题名: Quantitatively Mapping Cellular Viscosity with Detailed Organelle Information via a Designed PET Fluorescent Probe
作者: Liu, Tianyu1, 2;  Liu, Xiaogang3;  Spring, David R.4;  Qian, Xuhong5;  Cui, Jingnan2;  Xu, Zhaochao1
通讯作者: 徐兆超 ;  崔京南 ;  DavidSpring
刊名: SCIENTIFIC REPORTS
发表日期: 2014-06-24
DOI: 10.1038/srep05418
卷: 4, 期:1, 页:05418
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
英文摘要: Viscosity is a fundamental physical parameter that influences diffusion in biological processes. The distribution of intracellular viscosity is highly heterogeneous, and it is challenging to obtain a full map of cellular viscosity with detailed organelle information. In this work, we report 1 as the first fluorescent viscosity probe which is able to quantitatively map cellular viscosity with detailed organelle information based on the PET mechanism. This probe exhibited a significant ratiometric fluorescence intensity enhancement as solvent viscosity increases. The emission intensity increase was attributed to combined effects of the inhibition of PET due to restricted conformational access (favorable for FRET, but not for PET), and the decreased PET efficiency caused by viscosity-dependent twisted intramolecular charge transfer (TICT). A full map of subcellular viscosity was successfully constructed via fluorescent ratiometric detection and fluorescence lifetime imaging; it was found that lysosomal regions in a cell possess the highest viscosity, followed by mitochondrial regions.
关键词[WOS]: PHOTOINDUCED ELECTRON-TRANSFER ;  NITRIC-OXIDE SYNTHASE ;  MOLECULAR ROTORS ;  LIVE CELLS ;  CONFORMATIONAL DYNAMICS ;  MEMBRANE VISCOSITY ;  EXCITED-STATE ;  LIVING CELLS ;  SENSORS ;  DENSITY
语种: 英语
WOS记录号: WOS:000337889600002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/143917
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China
3.Univ Cambridge, Dept Phys, Cavendish Lab, Cambridge CB2 1TN, England
4.Univ Cambridge, Dept Chem, Cambridge CB2 1TN, England
5.E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China

Recommended Citation:
Liu, Tianyu,Liu, Xiaogang,Spring, David R.,et al. Quantitatively Mapping Cellular Viscosity with Detailed Organelle Information via a Designed PET Fluorescent Probe[J]. SCIENTIFIC REPORTS,2014,4(1):05418.
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