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学科主题: 物理化学
题名: Regulation of cell migration and osteogenic differentiation in mesenchymal stem cells under extremely low fluidic shear stress
作者: Gao, Xinghua1, 2;  Zhang, Xu1;  Xu, Hui1;  Zhou, Bingpu3;  Wen, Weijia2, 3;  Qin, Jianhua1
通讯作者: 秦建华
刊名: BIOMICROFLUIDICS
发表日期: 2014-09-01
DOI: 10.1063/1.4896557
卷: 8, 期:5, 页:052008
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Biophysics ;  Nanoscience & Nanotechnology ;  Physics, Fluids & Plasmas
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biophysics ;  Science & Technology - Other Topics ;  Physics
英文摘要: Mesenchymal stem cells (MSCs) are multipotent stem cells predominantly obtained from bone marrow, which are sensitive to mechanical loadings in physiological microenvironment. However, how the MSCs sense and respond to extremely low fluidic shear stress analogous to interstitial flow in vivo is poorly understood. In this work, we present a functional microfluidic device to examine the migration and differentiation behaviors of MSCs in response to multiple orders of physiologically relevant interstitial flow levels. The different magnitudes of fluid flow-induced shear stress were produced by a hydraulic resistance-based microfluidic perfusion system consisting of a microchannel network and a parallel of uniform cell culture chambers. By changing the length and width of the flow-in channels, the multiple magnitudes of low shear stresses could be generated ranging from similar to 10(-5) to similar to 10(-2) dyne/cm(2). We demonstrated enhanced significant F-actin expression and cell migration in MSCs under applied fluidic shear stress at similar to 10(-2) dyne/cm(2). We also demonstrated a significant osteogenic differentiation under this interstitial level of slow flows from similar to 10(-2) to similar to 10(-4) dyne/cm(2) in MSCs by analyzing alkaline phosphatase activity and osteopontin staining. Moreover, cytochalasin D and Rho-inhibitor Y-27632 significantly reduced the cytoskeleton F-actin expression and osteogenic differentiation in MSCs, indicating the mediated mechanical responses of MSCs under extremely low fluidic shear stress, possibly as a consequence of Rho-associated kinase pathway. The established microfluidic perfusion system with multiple shear-flow capabilities is simple and easy to operate, providing a flexible platform for studying the responses of diverse types of cells to the multiple interstitial flow levels in a single assay. (C) 2014 AIP Publishing LLC.
关键词[WOS]: BONE-MARROW ;  SIGNALING PATHWAYS ;  DYNAMIC CULTURE ;  FLOW ;  MICROFLUIDICS ;  PROLIFERATION ;  BIOREACTORS
语种: 英语
WOS记录号: WOS:000344226200010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/143946
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Hong Kong Univ Sci & Technol, Med Ctr, Shenzhen Peking Univ, Biomed Res Inst, Shenzhen 518000, Peoples R China
3.Hong Kong Univ Sci & Technol, Micro Nanofluid Joint Lab, Kowloon, Hong Kong, Peoples R China

Recommended Citation:
Gao, Xinghua,Zhang, Xu,Xu, Hui,et al. Regulation of cell migration and osteogenic differentiation in mesenchymal stem cells under extremely low fluidic shear stress[J]. BIOMICROFLUIDICS,2014,8(5):052008.
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