中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
学科主题: 物理化学
题名: A metabolomics-based method for studying the effect of yfcC gene in Escherichia coli on metabolism
作者: Wang, Xiyue1;  Xie, Yuping2;  Gao, Peng1;  Zhang, Sufang3;  Tan, Haidong3;  Yang, Fengxu2;  Lian, Rongwei2;  Tian, Jing2;  Xu, Guowang1
通讯作者: 高鹏 ;  田晶
关键词: Metabolomics ;  Gas chromatography-mass spectrometry ;  Escherichia coli ;  yfcC ;  Metabolic profiling
刊名: ANALYTICAL BIOCHEMISTRY
发表日期: 2014-04-15
DOI: 10.1016/j.ab.2014.01.018
卷: 451, 期:1, 页:48-55
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Biochemistry & Molecular Biology ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Metabolomics is a potent tool to assist in identifying the function of unknown genes through analysis of metabolite changes in the context of varied genetic backgrounds. However, the availability of a universal unbiased profiling analysis is still a big challenge. In this study, we report an optimized metabolic profiling method based on gas chromatography-mass spectrometry for Escherichia coli. It was found that physiological saline at -80 degrees C could ensure satisfied metabolic quenching with less metabolite leakage. A solution of methanol/water (21:79, v/v) was proved to be efficient for intracellular metabolite extraction. This method was applied to investigate the metabolome difference among wild-type E. coli, its yfcC deletion, and overexpression mutants. Statistical and bioinformatic analysis of the metabolic profiling data indicated that the expression of yfcC potentially affected the metabolism of glyoxylate shunt. This finding was further validated by real-time quantitative polymerase chain reactions showing that expression of aceA and aceB, the key genes in glyoxylate shunt, was upregulated by yfcC. This study exemplifies the robustness of the proposed metabolic profiling analysis strategy and its potential roles in investigating unknown gene functions in view of metabolome difference. (C) 2014 Elsevier Inc. All rights reserved.
关键词[WOS]: FUNCTIONAL GENOMICS TOOL ;  MASS-SPECTROMETRY ;  MICROBIAL METABOLOMICS ;  OPTIMIZATION ;  BACTERIA ;  IMPACT ;  K-12 ;  RECONSTRUCTION ;  METABOANALYST ;  ANNOTATION
语种: 英语
WOS记录号: WOS:000334144200011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/144013
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
2014LShyziHZ7y.PDF(1408KB)期刊论文作者接受稿开放获取View 联系获取全文

作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Dalian Polytech Univ, Sch Bioengn, Dalian 116034, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China

Recommended Citation:
Wang, Xiyue,Xie, Yuping,Gao, Peng,et al. A metabolomics-based method for studying the effect of yfcC gene in Escherichia coli on metabolism[J]. ANALYTICAL BIOCHEMISTRY,2014,451(1):48-55.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Wang, Xiyue]'s Articles
 [Xie, Yuping]'s Articles
 [Gao, Peng]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Wang, Xiyue]‘s Articles
 [Xie, Yuping]‘s Articles
 [Gao, Peng]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
文件名: 2014LShyziHZ7y.PDF
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace