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学科主题: 物理化学
题名: A Sub-Element in PRE enhances nuclear export of intronless mRNAs by recruiting the TREX complex via ZC3H18
作者: Chi, Binkai1;  Wang, Ke1;  Du, Yanhua1;  Gui, Bin1;  Chang, Xingya1;  Wang, Lantian1;  Fan, Jing1;  Chen, She2;  Wu, Xudong3;  Li, Guohui3;  Cheng, Hong1
通讯作者: 李国辉
刊名: NUCLEIC ACIDS RESEARCH
发表日期: 2014
DOI: 10.1093/nar/gku350
卷: 42, 期:11, 页:7305-7318
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Viral RNA elements that facilitate mRNA export are useful tools for identifying cellular RNA export factors. Here we show that hepatitis B virus post-transcriptional element (PRE) is one such element, and using PRE several new cellular mRNA export factors were identified. We found that PRE drastically enhances the cytoplasmic accumulation of cDNA transcripts independent of any viral protein. Systematic deletion analysis revealed the existence of a 116 nt functional Sub-Element of PRE (SEP1). The RNP that forms on the SEP1 RNA was affinity purified, in which TREX components as well as several other proteins were identified. TREX components and the SEP1-associating protein ZC3H18 are required for SEP1-mediated mRNA export. Significantly, ZC3H18 directly binds to the SEP1 RNA, interacts with TREX and is required for stable association of TREX with the SEP1-containing mRNA. Requirements for SEP1-mediated mRNA export are similar to those for splicing-dependent mRNA export. Consistent with these similarities, several SEP1-interacting proteins, including ZC3H18, ARS2, Acinus and Brr2, are required for efficient nuclear export of polyA RNAs. Together, our data indicate that SEP1 enhances mRNA export by recruiting TREX via ZC3H18. The new mRNA export factors that we identified might be involved in cap- and splicing-dependent TREX recruitment to cellular mRNAs.
关键词[WOS]: POSTTRANSCRIPTIONAL REGULATORY ELEMENT ;  CAP-BINDING COMPLEX ;  PROTEOMIC ANALYSIS ;  VIRUS TYPE-1 ;  THO COMPLEX ;  PROTEIN ;  TRANSCRIPTION ;  COMPONENTS ;  REV ;  POLYADENYLATION
语种: 英语
WOS记录号: WOS:000338769400049
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/144018
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai Key Lab Mol Androl,State Key Lab Mol Bio, Shanghai 200031, Peoples R China
2.Natl Inst Biol Sci, Beijing 102206, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Lab Mol Modeling & Design, Dalian 116023, Peoples R China

Recommended Citation:
Chi, Binkai,Wang, Ke,Du, Yanhua,et al. A Sub-Element in PRE enhances nuclear export of intronless mRNAs by recruiting the TREX complex via ZC3H18[J]. NUCLEIC ACIDS RESEARCH,2014,42(11):7305-7318.
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