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学科主题: 物理化学
题名: Identification of UshA as a major enzyme for NAD degradation in Escherichia coli
作者: Wang, Lei1, 4;  Zhou, Yongjin J.1;  Ji, Debin1;  Lin, Xinping1, 4;  Liu, Yuxue1, 4;  Zhang, Yixin1, 4;  Liu, Wujun1;  Zhao, Zongbao K.1, 2, 3
通讯作者: 赵宗保
关键词: Whole-cell biocatalysis ;  Nicotinamide adenine dinucleotide ;  NAD degradation ;  UshA ;  Pyrophosphatase ;  5 '-Nucleotidase
刊名: ENZYME AND MICROBIAL TECHNOLOGY
发表日期: 2014-05-10
DOI: 10.1016/j.enzmictec.2014.03.003
卷: 58-59, 期:1, 页:75-79
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: Nicotinamide adenine dinucleotide (NAD) and its reduced form NADH are essential cofactors for many redox biocatalysts. Because these cofactors are consumed in stoichiometric amounts, whole-cell biocatalysts have been routinely employed in order to reduce the costs. To further improve the efficacy of redox biocatalysts, it is essential to maintain the stability of nicotinamide cofactors, for which it is attractive to block degradation pathways for NAD(H). While the biosynthesis of NAD(H) has been well studied, it is less understood how NAD(H) are degraded. Here we demonstrated that UshA was a major periplasmic enzyme for NAD degradation in Escherichia colt. Purified recombinant UshA showed high pyrophosphatase activity with the catalytic efficiencies for hydrolysis of NAD and NADH at 3.7 mu M-1 s(-1) and 1.4 mu M-1 s(-1), respectively. Deletion of the ushA gene from the chromosome led to faster cell growth and improved extracellular NAD stability by 3-fold under conditions similar to whole-cell biocatalysis. These results significantly enriched our understanding on NAD metabolism, and should facilitate many applications including designing more robust redox biocatalysts. (c) 2014 Elsevier Inc. All rights reserved.
关键词[WOS]: HYDROLASE ;  CLONING ;  REDISTRIBUTION ;  AVAILABILITY ;  GENE
语种: 英语
WOS记录号: WOS:000336464600011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/144050
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian Natl Lab Clean Energy, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Wang, Lei,Zhou, Yongjin J.,Ji, Debin,et al. Identification of UshA as a major enzyme for NAD degradation in Escherichia coli[J]. ENZYME AND MICROBIAL TECHNOLOGY,2014,58-59(1):75-79.
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