DICP OpenIR
Subject Area物理化学
Functional integration of multiple genes into the genome of the oleaginous yeast Rhodosporidium toruloides
Lin, Xinping1,2; Wang, Yanan1,2; Zhang, Sufang1,3; Zhu, Zhiwei1,2; Zhou, Yongjin J.1; Yang, Fan4; Sun, Wenyi1; Wang, Xueying1; Zhao, Zongbao K.1,3; Zhao ZB(赵宗保)
KeywordRhodosporidium Toruloides Agrobacterium-mediated Transformation Gene Integration Antibiotic Resistance
Source PublicationFEMS YEAST RESEARCH
2014-06-01
DOI10.1111/1567-1364.12140
Volume14Issue:4Pages:547-555
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiotechnology & Applied Microbiology ; Microbiology ; Mycology
WOS Research AreaBiotechnology & Applied Microbiology ; Microbiology ; Mycology
WOS KeywordCRYPTOCOCCUS-NEOFORMANS ; LIPID PRODUCTION ; HOMOLOGOUS RECOMBINATION ; INSERTIONAL MUTAGENESIS ; MICROBIAL-PRODUCTION ; USTILAGO-MAYDIS ; RED YEASTS ; TRANSFORMATION ; AGROBACTERIUM ; FUNGI
AbstractThe basidiomycetous yeast Rhodosporidium toruloides represents an excellent producer for microbial lipids and carotenoids. However, further rational engineering of this unconventional yeast remains challenging partially because of the absence of efficient and reliable transformation method. In this study, we developed an Agrobacterium-mediated transformation (ATMT) protocol for effective gene integration into the R.toruloides genome. Both haploid and diploid strains were successfully modified, and the integration was confirmed by colony PCR, Western blot analysis and genome walking. We further demonstrated that multiple genes could be integrated by consecutive ATMT, leading to engineered strains simultaneously resistant to multiple antibiotics. Our results provided a practical method for functional integration and expression of exogenous genes in R.toruloides, which should facilitate the development of genetic tools and the construction of superior strains to produce biofuel molecules and biochemicals.
Language英语
WOS IDWOS:000337584500004
Citation statistics
Cited Times:40[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/144055
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhao ZB(赵宗保)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Dalian Natl Lab Clean Energy, Dalian, Peoples R China
4.Dalian Polytech Univ, Sch Biol Engn, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Lin, Xinping,Wang, Yanan,Zhang, Sufang,et al. Functional integration of multiple genes into the genome of the oleaginous yeast Rhodosporidium toruloides[J]. FEMS YEAST RESEARCH,2014,14(4):547-555.
APA Lin, Xinping.,Wang, Yanan.,Zhang, Sufang.,Zhu, Zhiwei.,Zhou, Yongjin J..,...&赵宗保.(2014).Functional integration of multiple genes into the genome of the oleaginous yeast Rhodosporidium toruloides.FEMS YEAST RESEARCH,14(4),547-555.
MLA Lin, Xinping,et al."Functional integration of multiple genes into the genome of the oleaginous yeast Rhodosporidium toruloides".FEMS YEAST RESEARCH 14.4(2014):547-555.
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