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学科主题: 物理化学
题名: Functional integration of multiple genes into the genome of the oleaginous yeast Rhodosporidium toruloides
作者: Lin, Xinping1, 2;  Wang, Yanan1, 2;  Zhang, Sufang1, 3;  Zhu, Zhiwei1, 2;  Zhou, Yongjin J.1;  Yang, Fan4;  Sun, Wenyi1;  Wang, Xueying1;  Zhao, Zongbao K.1, 3
通讯作者: 赵宗保
关键词: Rhodosporidium toruloides ;  Agrobacterium-mediated transformation ;  gene integration ;  antibiotic resistance
刊名: FEMS YEAST RESEARCH
发表日期: 2014-06-01
DOI: 10.1111/1567-1364.12140
卷: 14, 期:4, 页:547-555
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology ;  Microbiology ;  Mycology
研究领域[WOS]: Biotechnology & Applied Microbiology ;  Microbiology ;  Mycology
英文摘要: The basidiomycetous yeast Rhodosporidium toruloides represents an excellent producer for microbial lipids and carotenoids. However, further rational engineering of this unconventional yeast remains challenging partially because of the absence of efficient and reliable transformation method. In this study, we developed an Agrobacterium-mediated transformation (ATMT) protocol for effective gene integration into the R.toruloides genome. Both haploid and diploid strains were successfully modified, and the integration was confirmed by colony PCR, Western blot analysis and genome walking. We further demonstrated that multiple genes could be integrated by consecutive ATMT, leading to engineered strains simultaneously resistant to multiple antibiotics. Our results provided a practical method for functional integration and expression of exogenous genes in R.toruloides, which should facilitate the development of genetic tools and the construction of superior strains to produce biofuel molecules and biochemicals.
关键词[WOS]: CRYPTOCOCCUS-NEOFORMANS ;  LIPID PRODUCTION ;  HOMOLOGOUS RECOMBINATION ;  INSERTIONAL MUTAGENESIS ;  MICROBIAL-PRODUCTION ;  USTILAGO-MAYDIS ;  RED YEASTS ;  TRANSFORMATION ;  AGROBACTERIUM ;  FUNGI
语种: 英语
WOS记录号: WOS:000337584500004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/144055
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Dalian Natl Lab Clean Energy, Dalian, Peoples R China
4.Dalian Polytech Univ, Sch Biol Engn, Dalian, Peoples R China

Recommended Citation:
Lin, Xinping,Wang, Yanan,Zhang, Sufang,et al. Functional integration of multiple genes into the genome of the oleaginous yeast Rhodosporidium toruloides[J]. FEMS YEAST RESEARCH,2014,14(4):547-555.
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