DICP OpenIR
学科主题物理化学
Functional integration of multiple genes into the genome of the oleaginous yeast Rhodosporidium toruloides
Lin, Xinping1,2; Wang, Yanan1,2; Zhang, Sufang1,3; Zhu, Zhiwei1,2; Zhou, Yongjin J.1; Yang, Fan4; Sun, Wenyi1; Wang, Xueying1; Zhao, Zongbao K.1,3; Zhao ZB(赵宗保)
关键词Rhodosporidium Toruloides Agrobacterium-mediated Transformation Gene Integration Antibiotic Resistance
刊名FEMS YEAST RESEARCH
2014-06-01
DOI10.1111/1567-1364.12140
14期:4页:547-555
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology ; Microbiology ; Mycology
研究领域[WOS]Biotechnology & Applied Microbiology ; Microbiology ; Mycology
关键词[WOS]CRYPTOCOCCUS-NEOFORMANS ; LIPID PRODUCTION ; HOMOLOGOUS RECOMBINATION ; INSERTIONAL MUTAGENESIS ; MICROBIAL-PRODUCTION ; USTILAGO-MAYDIS ; RED YEASTS ; TRANSFORMATION ; AGROBACTERIUM ; FUNGI
英文摘要The basidiomycetous yeast Rhodosporidium toruloides represents an excellent producer for microbial lipids and carotenoids. However, further rational engineering of this unconventional yeast remains challenging partially because of the absence of efficient and reliable transformation method. In this study, we developed an Agrobacterium-mediated transformation (ATMT) protocol for effective gene integration into the R.toruloides genome. Both haploid and diploid strains were successfully modified, and the integration was confirmed by colony PCR, Western blot analysis and genome walking. We further demonstrated that multiple genes could be integrated by consecutive ATMT, leading to engineered strains simultaneously resistant to multiple antibiotics. Our results provided a practical method for functional integration and expression of exogenous genes in R.toruloides, which should facilitate the development of genetic tools and the construction of superior strains to produce biofuel molecules and biochemicals.
语种英语
WOS记录号WOS:000337584500004
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/144055
专题中国科学院大连化学物理研究所
通讯作者Zhao ZB(赵宗保)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Dalian Natl Lab Clean Energy, Dalian, Peoples R China
4.Dalian Polytech Univ, Sch Biol Engn, Dalian, Peoples R China
推荐引用方式
GB/T 7714
Lin, Xinping,Wang, Yanan,Zhang, Sufang,et al. Functional integration of multiple genes into the genome of the oleaginous yeast Rhodosporidium toruloides[J]. FEMS YEAST RESEARCH,2014,14(4):547-555.
APA Lin, Xinping.,Wang, Yanan.,Zhang, Sufang.,Zhu, Zhiwei.,Zhou, Yongjin J..,...&赵宗保.(2014).Functional integration of multiple genes into the genome of the oleaginous yeast Rhodosporidium toruloides.FEMS YEAST RESEARCH,14(4),547-555.
MLA Lin, Xinping,et al."Functional integration of multiple genes into the genome of the oleaginous yeast Rhodosporidium toruloides".FEMS YEAST RESEARCH 14.4(2014):547-555.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
2014RUkyoiaGYb.PDF(1164KB)期刊论文作者接受稿开放获取CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Lin, Xinping]的文章
[Wang, Yanan]的文章
[Zhang, Sufang]的文章
百度学术
百度学术中相似的文章
[Lin, Xinping]的文章
[Wang, Yanan]的文章
[Zhang, Sufang]的文章
必应学术
必应学术中相似的文章
[Lin, Xinping]的文章
[Wang, Yanan]的文章
[Zhang, Sufang]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。