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学科主题: 物理化学
题名: A simultaneous extraction method for metabolome and lipidome and its application in cry1Ac and sck-transgenic rice leaf treated with insecticide based on LC-MS analysis
作者: Chang, Yuwei1;  Zhang, Lei2;  Lu, Xin1;  Zhao, Chunxia1;  Zhu, Zhen2;  Wang, Feng3;  Zhang, Junjie1;  Chen, Shili1;  Zhao, Yanni1;  Xu, Guowang1
通讯作者: 许国旺
关键词: Methyl tert-butyl ether ;  Metabolomics ;  Lipidomics ;  Insecticide treatment ;  Transgenic rice
刊名: METABOLOMICS
发表日期: 2014-12-01
DOI: 10.1007/s11306-014-0658-6
卷: 10, 期:6, 页:1197-1209
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Endocrinology & Metabolism
研究领域[WOS]: Endocrinology & Metabolism
英文摘要: Abiotic stress caused by insecticide treatment is an interesting and challenging topic in plant research. Here a simultaneous extraction method with methyl tert-butyl ether for metabolomics and lipidomics analysis by using rapid resolution liquid chromatography quadrupole time-of-flight mass spectrometry was developed and validated. The extraction efficiency of lipidome and metabolome based on the current developed method showed an obvious improvement compared with literatures. About 300 metabolites were identified in rice leaf. Method validation was performed and analytical properties including the linearity (R-2, 0.991-1.000), repeatability (over 87 % peaks with CV < 20 % accounting for over 92 % of total response) and recovery (74.4-119.6 %) were satisfactory. The method was then applied to investigate time-course changes caused by insecticide treatment in transgenic rice with cry1Ac and sck genes and its wild counterpart. Antioxidants including ferulic acid and sinapic acid were down-regulated at 24 h after insecticide treatment. Signaling metabolites including salicylic acid and nicotinamide adenine dinucleotide were significantly up-regulated at 12-24 h after treatment in transgenic rice. More flavonoids were significantly changed in transgenic plants. Results indicated that gene transformation affected the metabolic response of rice to insecticide stress.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  TERT-BUTYL ETHER ;  MASS-SPECTROMETRY ;  METABONOMIC ANALYSIS ;  HPLC-MS ;  PLANTS ;  FLAVONOIDS ;  LEAVES ;  STRESS ;  ACID
语种: 英语
WOS记录号: WOS:000344555300016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/144197
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China
3.Fujian Acad Agr Sci, Fujian Prov Key Lab Genet Engn Agr, Fuzhou 350003, Peoples R China

Recommended Citation:
Chang, Yuwei,Zhang, Lei,Lu, Xin,et al. A simultaneous extraction method for metabolome and lipidome and its application in cry1Ac and sck-transgenic rice leaf treated with insecticide based on LC-MS analysis[J]. METABOLOMICS,2014,10(6):1197-1209.
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