DICP OpenIR
Subject Area物理化学
A simultaneous extraction method for metabolome and lipidome and its application in cry1Ac and sck-transgenic rice leaf treated with insecticide based on LC-MS analysis
Chang, Yuwei1; Zhang, Lei2; Lu, Xin1; Zhao, Chunxia1; Zhu, Zhen2; Wang, Feng3; Zhang, Junjie1; Chen, Shili1; Zhao, Yanni1; Xu, Guowang1; Xu GW(许国旺)
KeywordMethyl Tert-butyl Ether Metabolomics Lipidomics Insecticide Treatment Transgenic Rice
Source PublicationMETABOLOMICS
2014-12-01
DOI10.1007/s11306-014-0658-6
Volume10Issue:6Pages:1197-1209
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectEndocrinology & Metabolism
WOS Research AreaEndocrinology & Metabolism
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; TERT-BUTYL ETHER ; MASS-SPECTROMETRY ; METABONOMIC ANALYSIS ; HPLC-MS ; PLANTS ; FLAVONOIDS ; LEAVES ; STRESS ; ACID
AbstractAbiotic stress caused by insecticide treatment is an interesting and challenging topic in plant research. Here a simultaneous extraction method with methyl tert-butyl ether for metabolomics and lipidomics analysis by using rapid resolution liquid chromatography quadrupole time-of-flight mass spectrometry was developed and validated. The extraction efficiency of lipidome and metabolome based on the current developed method showed an obvious improvement compared with literatures. About 300 metabolites were identified in rice leaf. Method validation was performed and analytical properties including the linearity (R-2, 0.991-1.000), repeatability (over 87 % peaks with CV < 20 % accounting for over 92 % of total response) and recovery (74.4-119.6 %) were satisfactory. The method was then applied to investigate time-course changes caused by insecticide treatment in transgenic rice with cry1Ac and sck genes and its wild counterpart. Antioxidants including ferulic acid and sinapic acid were down-regulated at 24 h after insecticide treatment. Signaling metabolites including salicylic acid and nicotinamide adenine dinucleotide were significantly up-regulated at 12-24 h after treatment in transgenic rice. More flavonoids were significantly changed in transgenic plants. Results indicated that gene transformation affected the metabolic response of rice to insecticide stress.
Language英语
WOS IDWOS:000344555300016
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/144197
Collection中国科学院大连化学物理研究所
Corresponding AuthorXu GW(许国旺)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China
3.Fujian Acad Agr Sci, Fujian Prov Key Lab Genet Engn Agr, Fuzhou 350003, Peoples R China
Recommended Citation
GB/T 7714
Chang, Yuwei,Zhang, Lei,Lu, Xin,et al. A simultaneous extraction method for metabolome and lipidome and its application in cry1Ac and sck-transgenic rice leaf treated with insecticide based on LC-MS analysis[J]. METABOLOMICS,2014,10(6):1197-1209.
APA Chang, Yuwei.,Zhang, Lei.,Lu, Xin.,Zhao, Chunxia.,Zhu, Zhen.,...&许国旺.(2014).A simultaneous extraction method for metabolome and lipidome and its application in cry1Ac and sck-transgenic rice leaf treated with insecticide based on LC-MS analysis.METABOLOMICS,10(6),1197-1209.
MLA Chang, Yuwei,et al."A simultaneous extraction method for metabolome and lipidome and its application in cry1Ac and sck-transgenic rice leaf treated with insecticide based on LC-MS analysis".METABOLOMICS 10.6(2014):1197-1209.
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