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学科主题: 物理化学
题名: Proteomic analysis of protein methylation in the yeast Saccharomyces cerevisiae
作者: Wang, Keyun1, 2;  Zhou, Yongjin J.1;  Liu, Hongwei1;  Cheng, Kai1, 2;  Mao, Jiawei1, 2;  Wang, Fangjun1;  Liu, Wujun1;  Ye, Mingliang1;  Zhao, Zongbao K.1;  Zou, Hanfa1
通讯作者: 赵宗保 ;  邹汉法
关键词: Saccharomyces cerevisiae ;  Methylation ;  Proteomics ;  LC/MS/MS
刊名: JOURNAL OF PROTEOMICS
发表日期: 2015-01-30
DOI: 10.1016/j.jprot.2014.07.032
卷: 114, 期:1, 页:226-233
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Protein methylation catalyzed by SAM-dependent methyltransferase represents a major PTM involved in many important biological processes. Because methylation can occur on nitrogen, oxygen and sulfur centers and multiple methylation states exist on the nitrogen centers, methylproteome remains poorly documented. Here we present the methylation by isotope labeled SAM (MILS) strategy for a highly-confident analysis of the methylproteome of the yeast Saccharomyces cerevisiae based on the online multidimensional mu HPLC/MS/MS technology. We identified 43 methylated proteins, containing 68 methylation events associated with 64 methylation sites. More than 90% of these methylation events were previously unannotated in Uniprot database. Our results indicated, 1) over 2.6% of identified S. cerevisiae proteins are methylated, 2) the amino acid residue preference of protein methylation follows the order Lys Arg >> Asp > Asn approximate to Gln approximate to His > Glu > Cys, and 3) the methylation state on nitrogen center is largely exclusive. As our dataset covers various types of methylation centers, it provides rich information about yeast methylproteome and should significantly contribute to the field of protein methylaidon.
关键词[WOS]: ARGININE METHYLATION ;  LYSINE-METHYLATION ;  MASS-SPECTROMETRY ;  POSTTRANSLATIONAL MODIFICATIONS ;  S-ADENOSYLMETHIONINE ;  IN-VIVO ;  IDENTIFICATION ;  METHIONINE ;  SCALE ;  TRANSLATION
语种: 英语
WOS记录号: WOS:000348949100018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/144284
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Wang, Keyun,Zhou, Yongjin J.,Liu, Hongwei,et al. Proteomic analysis of protein methylation in the yeast Saccharomyces cerevisiae[J]. JOURNAL OF PROTEOMICS,2015,114(1):226-233.
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