DICP OpenIR
Subject Area物理化学
Proteomic analysis of protein methylation in the yeast Saccharomyces cerevisiae
Wang, Keyun1,2; Zhou, Yongjin J.1; Liu, Hongwei1; Cheng, Kai1,2; Mao, Jiawei1,2; Wang, Fangjun1; Liu, Wujun1; Ye, Mingliang1; Zhao, Zongbao K.1; Zou, Hanfa1; Zhao ZB(赵宗保); Zou HF(邹汉法)
KeywordSaccharomyces Cerevisiae Methylation Proteomics Lc/ms/ms
Source PublicationJOURNAL OF PROTEOMICS
2015-01-30
DOI10.1016/j.jprot.2014.07.032
Volume114Issue:1Pages:226-233
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemical Research Methods
WOS Research AreaBiochemistry & Molecular Biology
WOS KeywordARGININE METHYLATION ; LYSINE-METHYLATION ; MASS-SPECTROMETRY ; POSTTRANSLATIONAL MODIFICATIONS ; S-ADENOSYLMETHIONINE ; IN-VIVO ; IDENTIFICATION ; METHIONINE ; SCALE ; TRANSLATION
AbstractProtein methylation catalyzed by SAM-dependent methyltransferase represents a major PTM involved in many important biological processes. Because methylation can occur on nitrogen, oxygen and sulfur centers and multiple methylation states exist on the nitrogen centers, methylproteome remains poorly documented. Here we present the methylation by isotope labeled SAM (MILS) strategy for a highly-confident analysis of the methylproteome of the yeast Saccharomyces cerevisiae based on the online multidimensional mu HPLC/MS/MS technology. We identified 43 methylated proteins, containing 68 methylation events associated with 64 methylation sites. More than 90% of these methylation events were previously unannotated in Uniprot database. Our results indicated, 1) over 2.6% of identified S. cerevisiae proteins are methylated, 2) the amino acid residue preference of protein methylation follows the order Lys Arg >> Asp > Asn approximate to Gln approximate to His > Glu > Cys, and 3) the methylation state on nitrogen center is largely exclusive. As our dataset covers various types of methylation centers, it provides rich information about yeast methylproteome and should significantly contribute to the field of protein methylaidon.
Language英语
WOS IDWOS:000348949100018
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/144284
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhao ZB(赵宗保); Zou HF(邹汉法)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wang, Keyun,Zhou, Yongjin J.,Liu, Hongwei,et al. Proteomic analysis of protein methylation in the yeast Saccharomyces cerevisiae[J]. JOURNAL OF PROTEOMICS,2015,114(1):226-233.
APA Wang, Keyun.,Zhou, Yongjin J..,Liu, Hongwei.,Cheng, Kai.,Mao, Jiawei.,...&邹汉法.(2015).Proteomic analysis of protein methylation in the yeast Saccharomyces cerevisiae.JOURNAL OF PROTEOMICS,114(1),226-233.
MLA Wang, Keyun,et al."Proteomic analysis of protein methylation in the yeast Saccharomyces cerevisiae".JOURNAL OF PROTEOMICS 114.1(2015):226-233.
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