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学科主题: 物理化学
题名: Sample preparation for mass spectrometric analysis of human serum N-glycans using hydrophilic interaction chromatography-based solid phase extraction
作者: Cao, Liwei1;  Zhang, Ye2;  Chen, Linlin2;  Shen, Aijin1;  Zhang, Xingwang3;  Ren, Shifang3;  Gu, Jianxin3;  Yu, Long1;  Liang, Xinmiao1
通讯作者: 于龙 ;  梁鑫淼
刊名: ANALYST
发表日期: 2014-09-21
DOI: 10.1039/c4an00660g
卷: 139, 期:18, 页:4538-4546
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Expression levels of N-linked glycans derived from human serum glycoproteins have been shown to change during the progression of many diseases. Generally, N-glycans released from human serum proteins co-exist with endogenous serum peptides, salts, and other contaminants. Effective removal of these contaminants is essential to obtain the glycan profile of human serum proteins. Here, we developed a sample preparation method for mass spectrometry (MS) analysis of N-linked glycans derived from human serum glycoproteins based on a zwitterionic hydrophilic material named Click TE-Cys. The high hydrophilicity of Click TE-Cys, resulting from its unique surface structure and charge distribution, facilitated removal of co-existing salts and endogenous serum peptides. Furthermore, the present enrichment approach was handled in parallel, thus saving time. Using this method, a total of 47 unique N-glycans released from human serum proteins were identified. The intrabatch and interbatch coefficients of variation for the 47 N-linked glycans were 8.57% +/- 0.96% and 9.22% +/- 1.03%, respectively. These results demonstrate that the present method is suitable for fast purification of N-linked glycans derived from human serum glycoproteins, and has potential for clinical application.
关键词[WOS]: ORDERED MESOPOROUS CARBON ;  HUMAN PLASMA PROTEOME ;  LINKED GLYCANS ;  SELECTIVE ENRICHMENT ;  OVARIAN-CANCER ;  GLYCOMICS ;  GLYCOSYLATION ;  GLYCOPEPTIDES ;  DEGLYCOSYLATION ;  IDENTIFICATION
语种: 英语
WOS记录号: WOS:000340755000018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/144355
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 2, Dalian 116044, Peoples R China
3.Fudan Univ, Dept Biochem & Mol Biol, Key Lab Glycoconjugate Res, Minist Publ Hlth,Sch Basic Med Sci, Shanghai 200032, Peoples R China

Recommended Citation:
Cao, Liwei,Zhang, Ye,Chen, Linlin,et al. Sample preparation for mass spectrometric analysis of human serum N-glycans using hydrophilic interaction chromatography-based solid phase extraction[J]. ANALYST,2014,139(18):4538-4546.
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