DICP OpenIR
Subject Area物理化学
Sample preparation for mass spectrometric analysis of human serum N-glycans using hydrophilic interaction chromatography-based solid phase extraction
Cao, Liwei1; Zhang, Ye2; Chen, Linlin2; Shen, Aijin1; Zhang, Xingwang3; Ren, Shifang3; Gu, Jianxin3; Yu, Long1; Liang, Xinmiao1; Yu L(于龙); Liang XM(梁鑫淼)
Source PublicationANALYST
2014-09-21
DOI10.1039/c4an00660g
Volume139Issue:18Pages:4538-4546
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordORDERED MESOPOROUS CARBON ; HUMAN PLASMA PROTEOME ; LINKED GLYCANS ; SELECTIVE ENRICHMENT ; OVARIAN-CANCER ; GLYCOMICS ; GLYCOSYLATION ; GLYCOPEPTIDES ; DEGLYCOSYLATION ; IDENTIFICATION
AbstractExpression levels of N-linked glycans derived from human serum glycoproteins have been shown to change during the progression of many diseases. Generally, N-glycans released from human serum proteins co-exist with endogenous serum peptides, salts, and other contaminants. Effective removal of these contaminants is essential to obtain the glycan profile of human serum proteins. Here, we developed a sample preparation method for mass spectrometry (MS) analysis of N-linked glycans derived from human serum glycoproteins based on a zwitterionic hydrophilic material named Click TE-Cys. The high hydrophilicity of Click TE-Cys, resulting from its unique surface structure and charge distribution, facilitated removal of co-existing salts and endogenous serum peptides. Furthermore, the present enrichment approach was handled in parallel, thus saving time. Using this method, a total of 47 unique N-glycans released from human serum proteins were identified. The intrabatch and interbatch coefficients of variation for the 47 N-linked glycans were 8.57% +/- 0.96% and 9.22% +/- 1.03%, respectively. These results demonstrate that the present method is suitable for fast purification of N-linked glycans derived from human serum glycoproteins, and has potential for clinical application.
Language英语
WOS IDWOS:000340755000018
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/144355
Collection中国科学院大连化学物理研究所
Corresponding AuthorYu L(于龙); Liang XM(梁鑫淼)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 2, Dalian 116044, Peoples R China
3.Fudan Univ, Dept Biochem & Mol Biol, Key Lab Glycoconjugate Res, Minist Publ Hlth,Sch Basic Med Sci, Shanghai 200032, Peoples R China
Recommended Citation
GB/T 7714
Cao, Liwei,Zhang, Ye,Chen, Linlin,et al. Sample preparation for mass spectrometric analysis of human serum N-glycans using hydrophilic interaction chromatography-based solid phase extraction[J]. ANALYST,2014,139(18):4538-4546.
APA Cao, Liwei.,Zhang, Ye.,Chen, Linlin.,Shen, Aijin.,Zhang, Xingwang.,...&梁鑫淼.(2014).Sample preparation for mass spectrometric analysis of human serum N-glycans using hydrophilic interaction chromatography-based solid phase extraction.ANALYST,139(18),4538-4546.
MLA Cao, Liwei,et al."Sample preparation for mass spectrometric analysis of human serum N-glycans using hydrophilic interaction chromatography-based solid phase extraction".ANALYST 139.18(2014):4538-4546.
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