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学科主题物理化学
Enrichment and separation techniques for large-scale proteomics analysis of the protein post-translational modifications
Huang, Junfeng1,2; Wang, Fangjun1; Ye, Mingliang1; Zou, Hanfa1; Ye ML(叶明亮); Zou HF(邹汉法)
关键词Post-translational Modifications Enrichment Multi-dimensional Liquid Chromatography Separation Mass Spectrometry Protein Phosphorylation Protein Glycosylation
刊名JOURNAL OF CHROMATOGRAPHY A
2014-12-12
DOI10.1016/j.chroma.2014.10.107
1372期:1页:1-17
收录类别SCI
文章类型Review
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]HYDROPHILIC INTERACTION CHROMATOGRAPHY ; ION AFFINITY-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; SOLID-PHASE EXTRACTION ; N-LINKED GLYCOPEPTIDES ; MULTIDIMENSIONAL LIQUID-CHROMATOGRAPHY ; MONOLITHIC CAPILLARY COLUMN ; HIGHLY SELECTIVE ENRICHMENT ; GLUTATHIONYLATED CELLULAR-PROTEINS ; MULTIPLY PHOSPHORYLATED PEPTIDES
英文摘要Comprehensive analysis of the post-translational modifications (PTMs) on proteins at proteome level is crucial to elucidate the regulatory mechanisms of various biological processes. In the past decades, thanks to the development of specific PTM enrichment techniques and efficient multidimensional liquid chromatography (LC) separation strategy, the identification of protein PTMs have made tremendous progress. A huge number of modification sites for some major protein PTMs have been identified by proteomics analysis. In this review, we first introduced the recent progresses of PTM enrichment methods for the analysis of several major PTMs including phosphorylation, glycosylation, ubiquitination, acetylation, methylation, and oxidation/reduction status. We then briefly summarized the challenges for PTM enrichment. Finally, we introduced the fractionation and separation techniques for efficient separation of PTM peptides in large-scale PTM analysis. (C) 2014 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000347020900001
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/144392
专题中国科学院大连化学物理研究所
通讯作者Ye ML(叶明亮); Zou HF(邹汉法)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
2.Univ Chinese Acad Sci, Being, Peoples R China
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Huang, Junfeng,Wang, Fangjun,Ye, Mingliang,et al. Enrichment and separation techniques for large-scale proteomics analysis of the protein post-translational modifications[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1372(1):1-17.
APA Huang, Junfeng,Wang, Fangjun,Ye, Mingliang,Zou, Hanfa,叶明亮,&邹汉法.(2014).Enrichment and separation techniques for large-scale proteomics analysis of the protein post-translational modifications.JOURNAL OF CHROMATOGRAPHY A,1372(1),1-17.
MLA Huang, Junfeng,et al."Enrichment and separation techniques for large-scale proteomics analysis of the protein post-translational modifications".JOURNAL OF CHROMATOGRAPHY A 1372.1(2014):1-17.
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