中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
学科主题: 物理化学
题名: Enrichment and separation techniques for large-scale proteomics analysis of the protein post-translational modifications
作者: Huang, Junfeng1, 2;  Wang, Fangjun1;  Ye, Mingliang1;  Zou, Hanfa1
通讯作者: 叶明亮 ;  邹汉法
关键词: Post-translational modifications ;  Enrichment ;  Multi-dimensional liquid chromatography separation ;  Mass spectrometry ;  Protein phosphorylation ;  Protein glycosylation
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2014-12-12
DOI: 10.1016/j.chroma.2014.10.107
卷: 1372, 期:1, 页:1-17
收录类别: SCI
文章类型: Review
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Comprehensive analysis of the post-translational modifications (PTMs) on proteins at proteome level is crucial to elucidate the regulatory mechanisms of various biological processes. In the past decades, thanks to the development of specific PTM enrichment techniques and efficient multidimensional liquid chromatography (LC) separation strategy, the identification of protein PTMs have made tremendous progress. A huge number of modification sites for some major protein PTMs have been identified by proteomics analysis. In this review, we first introduced the recent progresses of PTM enrichment methods for the analysis of several major PTMs including phosphorylation, glycosylation, ubiquitination, acetylation, methylation, and oxidation/reduction status. We then briefly summarized the challenges for PTM enrichment. Finally, we introduced the fractionation and separation techniques for efficient separation of PTM peptides in large-scale PTM analysis. (C) 2014 Elsevier B.V. All rights reserved.
关键词[WOS]: HYDROPHILIC INTERACTION CHROMATOGRAPHY ;  ION AFFINITY-CHROMATOGRAPHY ;  TANDEM MASS-SPECTROMETRY ;  SOLID-PHASE EXTRACTION ;  N-LINKED GLYCOPEPTIDES ;  MULTIDIMENSIONAL LIQUID-CHROMATOGRAPHY ;  MONOLITHIC CAPILLARY COLUMN ;  HIGHLY SELECTIVE ENRICHMENT ;  GLUTATHIONYLATED CELLULAR-PROTEINS ;  MULTIPLY PHOSPHORYLATED PEPTIDES
语种: 英语
WOS记录号: WOS:000347020900001
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/144392
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
2014MM523kQ6CL.PDF(3823KB)期刊论文作者接受稿开放获取View Download

作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
2.Univ Chinese Acad Sci, Being, Peoples R China

Recommended Citation:
Huang, Junfeng,Wang, Fangjun,Ye, Mingliang,et al. Enrichment and separation techniques for large-scale proteomics analysis of the protein post-translational modifications[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1372(1):1-17.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Huang, Junfeng]'s Articles
 [Wang, Fangjun]'s Articles
 [Ye, Mingliang]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Huang, Junfeng]‘s Articles
 [Wang, Fangjun]‘s Articles
 [Ye, Mingliang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
文件名: 2014MM523kQ6CL.PDF
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace