DICP OpenIR
Subject Area物理化学
Enrichment and separation techniques for large-scale proteomics analysis of the protein post-translational modifications
Huang, Junfeng1,2; Wang, Fangjun1; Ye, Mingliang1; Zou, Hanfa1; Ye ML(叶明亮); Zou HF(邹汉法)
KeywordPost-translational Modifications Enrichment Multi-dimensional Liquid Chromatography Separation Mass Spectrometry Protein Phosphorylation Protein Glycosylation
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2014-12-12
DOI10.1016/j.chroma.2014.10.107
Volume1372Issue:1Pages:1-17
Indexed BySCI
SubtypeReview
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordHYDROPHILIC INTERACTION CHROMATOGRAPHY ; ION AFFINITY-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; SOLID-PHASE EXTRACTION ; N-LINKED GLYCOPEPTIDES ; MULTIDIMENSIONAL LIQUID-CHROMATOGRAPHY ; MONOLITHIC CAPILLARY COLUMN ; HIGHLY SELECTIVE ENRICHMENT ; GLUTATHIONYLATED CELLULAR-PROTEINS ; MULTIPLY PHOSPHORYLATED PEPTIDES
AbstractComprehensive analysis of the post-translational modifications (PTMs) on proteins at proteome level is crucial to elucidate the regulatory mechanisms of various biological processes. In the past decades, thanks to the development of specific PTM enrichment techniques and efficient multidimensional liquid chromatography (LC) separation strategy, the identification of protein PTMs have made tremendous progress. A huge number of modification sites for some major protein PTMs have been identified by proteomics analysis. In this review, we first introduced the recent progresses of PTM enrichment methods for the analysis of several major PTMs including phosphorylation, glycosylation, ubiquitination, acetylation, methylation, and oxidation/reduction status. We then briefly summarized the challenges for PTM enrichment. Finally, we introduced the fractionation and separation techniques for efficient separation of PTM peptides in large-scale PTM analysis. (C) 2014 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000347020900001
Citation statistics
Cited Times:50[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/144392
Collection中国科学院大连化学物理研究所
Corresponding AuthorYe ML(叶明亮); Zou HF(邹汉法)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
2.Univ Chinese Acad Sci, Being, Peoples R China
Recommended Citation
GB/T 7714
Huang, Junfeng,Wang, Fangjun,Ye, Mingliang,et al. Enrichment and separation techniques for large-scale proteomics analysis of the protein post-translational modifications[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1372(1):1-17.
APA Huang, Junfeng,Wang, Fangjun,Ye, Mingliang,Zou, Hanfa,叶明亮,&邹汉法.(2014).Enrichment and separation techniques for large-scale proteomics analysis of the protein post-translational modifications.JOURNAL OF CHROMATOGRAPHY A,1372(1),1-17.
MLA Huang, Junfeng,et al."Enrichment and separation techniques for large-scale proteomics analysis of the protein post-translational modifications".JOURNAL OF CHROMATOGRAPHY A 1372.1(2014):1-17.
Files in This Item:
File Name/Size DocType Version Access License
2014MM523kQ6CL.PDF(3823KB)期刊论文作者接受稿开放获取CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Huang, Junfeng]'s Articles
[Wang, Fangjun]'s Articles
[Ye, Mingliang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Huang, Junfeng]'s Articles
[Wang, Fangjun]'s Articles
[Ye, Mingliang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Huang, Junfeng]'s Articles
[Wang, Fangjun]'s Articles
[Ye, Mingliang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.