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学科主题: 物理化学
题名: Recent advances in stable isotope labeling based techniques for proteome relative quantification
作者: Zhou, Yuan1;  Shan, Yichu1;  Zhang, Lihua1;  Zhang, Yukui1
通讯作者: 张丽华
关键词: Proteome ;  Relative quantification ;  Stable isotope labeling ;  Metabolic labeling ;  Chemical labeling ;  Enzyme-catalyzed labeling
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2014-10-24
DOI: 10.1016/j.chroma.2014.08.098
卷: 1365, 期:1, 页:1-11
收录类别: SCI
文章类型: Review
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: The large scale relative quantification of all proteins expressed in biological samples under different states is of great importance for discovering proteins with important biological functions, as well as screening disease related biomarkers and drug targets. Therefore, the accurate quantification of proteins at proteome level has become one of the key issues in protein science. Herein, the recent advances in stable isotope labeling based techniques for proteome relative quantification were reviewed, from the aspects of metabolic labeling, chemical labeling and enzyme-catalyzed labeling. Furthermore, the future research direction in this field was prospected. (C) 2014 Elsevier B.V. All rights reserved.
关键词[WOS]: MULTIPLEXED QUANTITATIVE PROTEOMICS ;  CODED AFFINITY TAGS ;  MASS-SPECTROMETRY ;  ACCURATE QUANTITATION ;  CELL-CULTURE ;  AMINO-ACIDS ;  IN-VIVO ;  PEPTIDE ;  PROTEINS ;  SILAC
语种: 英语
WOS记录号: WOS:000343618600001
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/144413
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Zhou, Yuan,Shan, Yichu,Zhang, Lihua,et al. Recent advances in stable isotope labeling based techniques for proteome relative quantification[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1365(1):1-11.
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