DICP OpenIR
Subject Area物理化学
Identification of phosphopeptides with unknown cleavage specificity by a de novo sequencing assisted database search strategy
Dong, Mingming1,2; Ye, Mingliang1; Cheng, Kai1,2; Dong, Jing1; Zhu, Jun1,2; Qin, Hongqiang1,2; Bian, Yangyang1,2; Zou, Hanfa1; Ye ML(叶明亮); Zou HF(邹汉法)
KeywordDe Novo Sequencing Endogenous Phosphopeptide Phosphoproteomics Phosphopeptide Identification Technology
Source PublicationPROTEOMICS
2014-11-01
DOI10.1002/pmic.201400268
Volume14Issue:21-22Pages:2410-2416
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology
WOS Research AreaBiochemistry & Molecular Biology
WOS KeywordSCALE PHOSPHOPROTEOME ANALYSIS ; CHROMATOGRAPHY ; ENRICHMENT ; PEPTIDOME
AbstractIn theory, proteases with broad cleavage specificity could be applied to digest protein samples to improve the phosphoproteomic analysis coverage. However, in practice this approach is seldom employed. This is because the identification of phosphopeptides without enzyme specificity by conventional database search strategy is extremely difficult due to the huge search space. In this study, we investigated the performance of a de novo sequencing assisted database search strategy for the identification of such phosphopeptides. Firstly, we compared the performance of conventional database search strategy and the de novo sequencing assisted database search strategy for the identification of peptides and phosphopeptides without stetting enzyme specificity. It was found that the identification sensitivity dropped significantly for the conventional one while it was only slightly decreased for the new approach. Then, this new search strategy was applied to identify phosphopeptides generated by Proteinase K digestion, which resulted in the identification of 717 phosphopeptides. Finally, this strategy was utilized for the identification of serum endogenous phosphopeptides, which were generated in vivo by different kinds of proteases and kinases, and the identification of 68 unique serum endogenous phosphopepitdes was successfully achieved.
Language英语
WOS IDWOS:000344807600004
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/144419
Collection中国科学院大连化学物理研究所
Corresponding AuthorYe ML(叶明亮); Zou HF(邹汉法)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Dong, Mingming,Ye, Mingliang,Cheng, Kai,et al. Identification of phosphopeptides with unknown cleavage specificity by a de novo sequencing assisted database search strategy[J]. PROTEOMICS,2014,14(21-22):2410-2416.
APA Dong, Mingming.,Ye, Mingliang.,Cheng, Kai.,Dong, Jing.,Zhu, Jun.,...&邹汉法.(2014).Identification of phosphopeptides with unknown cleavage specificity by a de novo sequencing assisted database search strategy.PROTEOMICS,14(21-22),2410-2416.
MLA Dong, Mingming,et al."Identification of phosphopeptides with unknown cleavage specificity by a de novo sequencing assisted database search strategy".PROTEOMICS 14.21-22(2014):2410-2416.
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