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学科主题物理化学
Guanidinium functionalized superparamagnetic silica spheres for selective enrichment of phosphopeptides and intact phosphoproteins from complex mixtures
Deng, Qiliang1,2; Wu, Jianhua2; Chen, Yang1; Zhang, Zhijun2; Wang, Yang2; Fang, Guozhen2; Wang, Shuo2; Zhang, Yukui3; ShuoWang
刊名JOURNAL OF MATERIALS CHEMISTRY B
2014-02-28
DOI10.1039/c3tb21540g
2期:8页:1048-1058
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Technology
类目[WOS]Materials Science, Biomaterials
研究领域[WOS]Materials Science
关键词[WOS]METAL-AFFINITY-CHROMATOGRAPHY ; PHOSPHORYLATED PROTEINS ; MASS-SPECTROMETRY ; RECOGNITION ; PURIFICATION ; STRATEGIES ; PROTEOMICS ; RECEPTORS ; MECHANISM
英文摘要Phosphorylation of protein regulates nearly all biological processes in nature. The development of enrichment techniques for phosphorylated proteins is vital to systematic identification and characterization of phosphoproteins. Here, a general strategy for highly efficient capture of intact phosphorylated proteins from protein mixtures has been developed by using guanidine functionalized superparamagnetic microspheres (denoted as Fe3O4@SiO2@GDN). The Fe3O4@SiO2@GDN was prepared by modifying Fe3O4@SiO2 with 3-guanidopropyl triethoxysilane as a functionalization monomer. The resulting materials could specifically and selectively recognize phosphoproteins and showed high binding capacities for model phosphoproteins (78.8 mg g(-1) for ovalbumin (OVA) and 59.6 mg g(-1) for beta-casein (beta-Cas), respectively). The feasibility of the resulting material for phosphoprotein enrichment has also been demonstrated by selectively binding and capturing phosphoproteins from complex protein mixtures and real samples (milk, egg, and tissue protein extract from a mouse liver), respectively. In addition, the selective enrichment of phosphopeptides has also been investigated. The proposed technique showed application potential for phosphoprotein and phosphopeptide enrichment.
语种英语
WOS记录号WOS:000331500800012
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被引频次:32[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/144437
专题中国科学院大连化学物理研究所
通讯作者ShuoWang
作者单位1.Tianjin Univ Sci & Technol, Dept Sci, Tianjin 300457, Peoples R China
2.Tianjin Univ Sci & Technol, Key Lab Food Nutr & Safety, Minist Educ China, Tianjin 300457, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
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Deng, Qiliang,Wu, Jianhua,Chen, Yang,et al. Guanidinium functionalized superparamagnetic silica spheres for selective enrichment of phosphopeptides and intact phosphoproteins from complex mixtures[J]. JOURNAL OF MATERIALS CHEMISTRY B,2014,2(8):1048-1058.
APA Deng, Qiliang.,Wu, Jianhua.,Chen, Yang.,Zhang, Zhijun.,Wang, Yang.,...&ShuoWang.(2014).Guanidinium functionalized superparamagnetic silica spheres for selective enrichment of phosphopeptides and intact phosphoproteins from complex mixtures.JOURNAL OF MATERIALS CHEMISTRY B,2(8),1048-1058.
MLA Deng, Qiliang,et al."Guanidinium functionalized superparamagnetic silica spheres for selective enrichment of phosphopeptides and intact phosphoproteins from complex mixtures".JOURNAL OF MATERIALS CHEMISTRY B 2.8(2014):1048-1058.
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