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学科主题: 物理化学
题名: Guanidinium functionalized superparamagnetic silica spheres for selective enrichment of phosphopeptides and intact phosphoproteins from complex mixtures
作者: Deng, Qiliang1, 2;  Wu, Jianhua2;  Chen, Yang1;  Zhang, Zhijun2;  Wang, Yang2;  Fang, Guozhen2;  Wang, Shuo2;  Zhang, Yukui3
通讯作者: ShuoWang
刊名: JOURNAL OF MATERIALS CHEMISTRY B
发表日期: 2014-02-28
DOI: 10.1039/c3tb21540g
卷: 2, 期:8, 页:1048-1058
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Materials Science, Biomaterials
研究领域[WOS]: Materials Science
英文摘要: Phosphorylation of protein regulates nearly all biological processes in nature. The development of enrichment techniques for phosphorylated proteins is vital to systematic identification and characterization of phosphoproteins. Here, a general strategy for highly efficient capture of intact phosphorylated proteins from protein mixtures has been developed by using guanidine functionalized superparamagnetic microspheres (denoted as Fe3O4@SiO2@GDN). The Fe3O4@SiO2@GDN was prepared by modifying Fe3O4@SiO2 with 3-guanidopropyl triethoxysilane as a functionalization monomer. The resulting materials could specifically and selectively recognize phosphoproteins and showed high binding capacities for model phosphoproteins (78.8 mg g(-1) for ovalbumin (OVA) and 59.6 mg g(-1) for beta-casein (beta-Cas), respectively). The feasibility of the resulting material for phosphoprotein enrichment has also been demonstrated by selectively binding and capturing phosphoproteins from complex protein mixtures and real samples (milk, egg, and tissue protein extract from a mouse liver), respectively. In addition, the selective enrichment of phosphopeptides has also been investigated. The proposed technique showed application potential for phosphoprotein and phosphopeptide enrichment.
关键词[WOS]: METAL-AFFINITY-CHROMATOGRAPHY ;  PHOSPHORYLATED PROTEINS ;  MASS-SPECTROMETRY ;  RECOGNITION ;  PURIFICATION ;  STRATEGIES ;  PROTEOMICS ;  RECEPTORS ;  MECHANISM
语种: 英语
WOS记录号: WOS:000331500800012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/144437
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Tianjin Univ Sci & Technol, Dept Sci, Tianjin 300457, Peoples R China
2.Tianjin Univ Sci & Technol, Key Lab Food Nutr & Safety, Minist Educ China, Tianjin 300457, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Deng, Qiliang,Wu, Jianhua,Chen, Yang,et al. Guanidinium functionalized superparamagnetic silica spheres for selective enrichment of phosphopeptides and intact phosphoproteins from complex mixtures[J]. JOURNAL OF MATERIALS CHEMISTRY B,2014,2(8):1048-1058.
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