DICP OpenIR
Subject Area物理化学
Boronic acid functionalized magnetic nanoparticles via thiol-ene click chemistry for selective enrichment of glycoproteins
Zhang, Sitong1,2; He, Xiwen1,2; Chen, Langxing1,2; Zhang, Yukui1,2,3; Chen LX(陈朗星)
Source PublicationNEW JOURNAL OF CHEMISTRY
2014-09-01
DOI10.1039/c4nj00424h
Volume38Issue:9Pages:4212-4218
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordEFFICIENT STRATEGY ; GLYCOPEPTIDES ; CAPTURE ; PHOTOPOLYMERIZATION ; NETWORKS ; PROTEIN ; ALKYNES ; SAMPLES ; FACILE
AbstractA simple, novel approach was developed for the preparation of boronic acid functionalized Fe3O4 magnetic nanoparticles (MNPs) via thiol-ene (TE) click reaction. In this work, two clickable Fe3O4 MNPs functionalized with either alkene or thiol moieties were synthesized. Firstly, Fe3O4 MNPs were synthesized through a solvothermal method and then the clickable alkene- or thiol-coated Fe3O4 MNPs were prepared by sot gel reaction with an organosilicon coupling agent, 3-(methacryloyloxy) propyltrimethoxylsilane (MPS) or 3-mercaptopropyltriethoxysilane (MPTES). The carbon carbon double bonds/thiol groups on the surface of the MNPs serve as clickable sites to react with 4-mercaptophenylboronic acid (4-MPBA)/3-acrylamidophenylboronic acid (AAPBA) during the subsequent TE click reaction. Finally, the high density of boronic acid ligands immobilized on the surface of the Fe3O4 MNPs was obtained via TE click reactions. The morphology, adsorption and recognition properties of the Fe3O4 MNPs were investigated by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), vibrating sample magnetomety (VSM) and X-ray photoelectron spectrometry (XPS). Four proteins, including ovalbumin (OB) and transferrin (Trf) as glycoprotein templates, and lysozyme (Lyz) and horse heart cytochrome c (Cyt C) as non-glycoprotein templates are chosen as target proteins. Two types of click-Fe3O4 MNP (Fe3O4@MPS@PBA and Fe3O4@SH@AAPBA) exhibit a high binding capacity and excellent specificity towards glycoproteins, and can selectively capture and separate glycoproteins from egg white samples directly. Furthermore, this work could provide a promising method of surface modification for the design of more efficient adsorbents for the isolation and enrichment of proteins from complex bio-samples.
Language英语
WOS IDWOS:000341065800024
Citation statistics
Cited Times:42[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/144442
Collection中国科学院大连化学物理研究所
Corresponding AuthorChen LX(陈朗星)
Affiliation1.Nankai Univ, Coll Chem, Res Ctr Analyt Sci, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
2.Collaborat Innovat Ctr Chem Sci & Engn, Tianjin, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Sitong,He, Xiwen,Chen, Langxing,et al. Boronic acid functionalized magnetic nanoparticles via thiol-ene click chemistry for selective enrichment of glycoproteins[J]. NEW JOURNAL OF CHEMISTRY,2014,38(9):4212-4218.
APA Zhang, Sitong,He, Xiwen,Chen, Langxing,Zhang, Yukui,&陈朗星.(2014).Boronic acid functionalized magnetic nanoparticles via thiol-ene click chemistry for selective enrichment of glycoproteins.NEW JOURNAL OF CHEMISTRY,38(9),4212-4218.
MLA Zhang, Sitong,et al."Boronic acid functionalized magnetic nanoparticles via thiol-ene click chemistry for selective enrichment of glycoproteins".NEW JOURNAL OF CHEMISTRY 38.9(2014):4212-4218.
Files in This Item:
File Name/Size DocType Version Access License
2014NEEUtTCIvZ.PDF(2348KB)期刊论文作者接受稿开放获取CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Sitong]'s Articles
[He, Xiwen]'s Articles
[Chen, Langxing]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Sitong]'s Articles
[He, Xiwen]'s Articles
[Chen, Langxing]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Sitong]'s Articles
[He, Xiwen]'s Articles
[Chen, Langxing]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.