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学科主题: 物理化学
题名: The Synthesis of Magnetic Lysozyme-Imprinted Polymers by Means of Distillation-Precipitation Polymerization for Selective Protein Enrichment
作者: Cao, Jiali1, 3;  Zhang, Xihao1, 3;  He, Xiwen1;  Chen, Langxing1;  Zhang, Yukui1, 2, 3
通讯作者: JialiCao
关键词: imprinting ;  magnetic properties ;  nanoparticles ;  polymerization ;  proteins
刊名: CHEMISTRY-AN ASIAN JOURNAL
发表日期: 2014-02-01
DOI: 10.1002/asia.201300937
卷: 9, 期:2, 页:526-533
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: A protein imprinting approach for the synthesis of core-shell structure nanoparticles with a magnetic core and molecularly imprinted polymer (MIP) shell was developed using a simple distillation-precipitation polymerization method. In this work, Fe3O4 magnetic nanoparticles were first synthesized through a solvothermal method and then were conveniently surface-modified with 3-(methacryloyloxy)propyltrimethoxylsilane as anchor molecules to donate vinyl groups. Next a high-density MIP shell was coated onto the surface of the magnetic nanoparticles by the copolymerization of functional monomer acrylamide (AAm), cross-linking agent N,N-methylenebisacrylamide (MBA), the initiator azodiisobutyronitrile (AIBN), and protein in acetonitrile heated at reflux. The morphology, adsorption, and recognition properties of the magnetic molecularly imprinted nanoparticles were investigated by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), vibrating sample magnetometer (VSM), and rebinding experiments. The resulting MIP showed a high adsorption capacity (104.8mgg(-1)) and specific recognition (imprinting factor=7.6) to lysozyme (Lyz). The as-prepared Fe3O4@Lyz-MIP nanoparticles with a mean diameter of 320nm were coated with an MIP shell that was 20nm thick, which enabled Fe3O4@Lyz-MIP to easily reach adsorption equilibrium. The high magnetization saturation (40.35emug(-1)) endows the materials with the convenience of magnetic separation under an external magnetic field and allows them to be subsequently reused. Furthermore, Fe3O4@Lyz-MIP could selectively extract a target protein from real egg-white samples under an external magnetic field.
关键词[WOS]: SOLID-PHASE EXTRACTION ;  HISTIDINE-TAGGED PROTEINS ;  TOF-MS ANALYSIS ;  RIBONUCLEASE-A ;  SUBMICROMETER PARTICLES ;  HIGHLY EFFICIENT ;  AQUEOUS-MEDIA ;  NANOPARTICLES ;  MICROSPHERES ;  RECOGNITION
语种: 英语
WOS记录号: WOS:000331088600020
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/144456
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, Dept Chem, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Synerget Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China

Recommended Citation:
Cao, Jiali,Zhang, Xihao,He, Xiwen,et al. The Synthesis of Magnetic Lysozyme-Imprinted Polymers by Means of Distillation-Precipitation Polymerization for Selective Protein Enrichment[J]. CHEMISTRY-AN ASIAN JOURNAL,2014,9(2):526-533.
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