DICP OpenIR
Subject Area物理化学
Preparation of protein imprinted materials by hierarchical imprinting techniques and application in selective depletion of albumin from human serum
Liu, Jinxiang1,2; Deng, Qiliang1; Tao, Dingyin1,2; Yang, Kaiguang1; Zhang, Lihua1; Liang, Zhen1; Zhang, Yukui1; Zhang LH(张丽华)
Source PublicationSCIENTIFIC REPORTS
2014-06-30
DOI10.1038/srep05487
Volume4Issue:1Pages:1
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology
WOS SubjectMultidisciplinary Sciences
WOS Research AreaScience & Technology - Other Topics
WOS KeywordPOLYMERS ; NANOPARTICLES ; RECOGNITION ; PROTEOMICS ; SAMPLES ; WATER
AbstractHierarchical imprinting was developed to prepare the protein imprinted materials, as the artificial antibody, for the selective depletion of HSA from the human serum proteome. Porcine serum albumin (PSA) was employed as the dummy template for the fabrication of the recognition sites. To demonstrate the advantages of the hierarchical imprinting, molecularly imprinted polymers prepared by hierarchical imprinting technique (h-MIPs) were compared with those obtained by bulk imprinting (b-MIPs), in terms of the binding capacity, adsorption kinetics, selectivity and synthesis reproducibility. The binding capacity of h-MIPs could reach 12 mg g(-1). And saturation binding could be reached in less than 20 min for the h-MIPs. In the protein mixture, h-MIPs exhibit excellent selectivity for PSA, with imprinting factors as about 3.6, much higher than those for non-template proteins. For the proteomic application, the identified protein group number in serum treated by h-MIPs was increased to 422, which is 21% higher than that obtained from the original serum, meanwhile the identified protein group number for the Albumin Removal kit was only 376. The results demonstrate that protein imprinted polymers prepared by hierarchical imprinting technique, might become the artificial antibodies for the selective depletion of high abundance proteins in proteome study.
Language英语
WOS IDWOS:000338103700003
Citation statistics
Cited Times:32[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/144458
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang LH(张丽华)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Liu, Jinxiang,Deng, Qiliang,Tao, Dingyin,et al. Preparation of protein imprinted materials by hierarchical imprinting techniques and application in selective depletion of albumin from human serum[J]. SCIENTIFIC REPORTS,2014,4(1):1.
APA Liu, Jinxiang.,Deng, Qiliang.,Tao, Dingyin.,Yang, Kaiguang.,Zhang, Lihua.,...&张丽华.(2014).Preparation of protein imprinted materials by hierarchical imprinting techniques and application in selective depletion of albumin from human serum.SCIENTIFIC REPORTS,4(1),1.
MLA Liu, Jinxiang,et al."Preparation of protein imprinted materials by hierarchical imprinting techniques and application in selective depletion of albumin from human serum".SCIENTIFIC REPORTS 4.1(2014):1.
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