DICP OpenIR
Subject Area物理化学
1-Dodecyl-3-Methylimidazolium Chloride-Assisted Sample Preparation Method for Efficient Integral Membrane Proteome Analysis
Zhao, Qun1,2; Fang, Fei1,2; Liang, Yu1; Yuan, Huiming1; Yang, Kaiguang1; Wu, Qi1,2; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1; Zhang LH(张丽华)
Source PublicationANALYTICAL CHEMISTRY
2014-08-05
DOI10.1021/ac5013267
Volume86Issue:15Pages:7544-7550
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordTANDEM MASS-SPECTROMETRY ; IONIC LIQUIDS ; TRYPTIC DIGESTION ; INFRARED-SPECTROSCOPY ; LC-MS/MS ; PROTEINS ; SOLUBILIZATION ; METHANOL ; IDENTIFICATION ; CHROMATOGRAPHY
AbstractDue to their extremely hydrophobic nature, the analysis of integral membrane proteins (IMPs) is of great challenge. Although various additives have been applied to improve the solubility of IMPs, they still suffer from low solubilization efficiency, incompatibility with trypsin digestion, or interference with MS detection. Herein, the systematic study on the effect of ionic liquid structure on membrane protein solubilization and trypsin biocompatibility was performed, based on which 1-dodecyl-3-methylimidazolium chloride (C12Im-Cl) was selected for the sample preparation of IMPs. Compared with other commonly used additives, such as sodium dodecyl sulfate (SDS), Rapigest, and methanol, C12Im-Cl showed the best performance. In addition, with a strong cation exchange trap column, it could be easily removed after trypsin digestion, which not only was beneficial to avoid protein precipitation during digestion but also had no adverse effect on LC-MS-based separation and detection. Such a C12Im-Cl-assisted sample preparation method was further applied to the membrane proteome analysis of rat brain. Compared with the SDS-assisted method, 1.4 and 3.5 times improvement on the identified IMP and hydrophobic peptide number were achieved (251 vs 178, and 982 vs 279). All these results demonstrated that the C12Im-Cl-assisted sample preparation method is of great promise to promote the large-scale membrane proteome profiling.
Language英语
WOS IDWOS:000340081100057
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/144459
Collection中国科学院大连化学物理研究所
Corresponding AuthorZhang LH(张丽华)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Qun,Fang, Fei,Liang, Yu,et al. 1-Dodecyl-3-Methylimidazolium Chloride-Assisted Sample Preparation Method for Efficient Integral Membrane Proteome Analysis[J]. ANALYTICAL CHEMISTRY,2014,86(15):7544-7550.
APA Zhao, Qun.,Fang, Fei.,Liang, Yu.,Yuan, Huiming.,Yang, Kaiguang.,...&张丽华.(2014).1-Dodecyl-3-Methylimidazolium Chloride-Assisted Sample Preparation Method for Efficient Integral Membrane Proteome Analysis.ANALYTICAL CHEMISTRY,86(15),7544-7550.
MLA Zhao, Qun,et al."1-Dodecyl-3-Methylimidazolium Chloride-Assisted Sample Preparation Method for Efficient Integral Membrane Proteome Analysis".ANALYTICAL CHEMISTRY 86.15(2014):7544-7550.
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