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学科主题物理化学
1-Dodecyl-3-Methylimidazolium Chloride-Assisted Sample Preparation Method for Efficient Integral Membrane Proteome Analysis
Zhao, Qun1,2; Fang, Fei1,2; Liang, Yu1; Yuan, Huiming1; Yang, Kaiguang1; Wu, Qi1,2; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1; Zhang LH(张丽华)
刊名ANALYTICAL CHEMISTRY
2014-08-05
DOI10.1021/ac5013267
86期:15页:7544-7550
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]TANDEM MASS-SPECTROMETRY ; IONIC LIQUIDS ; TRYPTIC DIGESTION ; INFRARED-SPECTROSCOPY ; LC-MS/MS ; PROTEINS ; SOLUBILIZATION ; METHANOL ; IDENTIFICATION ; CHROMATOGRAPHY
英文摘要Due to their extremely hydrophobic nature, the analysis of integral membrane proteins (IMPs) is of great challenge. Although various additives have been applied to improve the solubility of IMPs, they still suffer from low solubilization efficiency, incompatibility with trypsin digestion, or interference with MS detection. Herein, the systematic study on the effect of ionic liquid structure on membrane protein solubilization and trypsin biocompatibility was performed, based on which 1-dodecyl-3-methylimidazolium chloride (C12Im-Cl) was selected for the sample preparation of IMPs. Compared with other commonly used additives, such as sodium dodecyl sulfate (SDS), Rapigest, and methanol, C12Im-Cl showed the best performance. In addition, with a strong cation exchange trap column, it could be easily removed after trypsin digestion, which not only was beneficial to avoid protein precipitation during digestion but also had no adverse effect on LC-MS-based separation and detection. Such a C12Im-Cl-assisted sample preparation method was further applied to the membrane proteome analysis of rat brain. Compared with the SDS-assisted method, 1.4 and 3.5 times improvement on the identified IMP and hydrophobic peptide number were achieved (251 vs 178, and 982 vs 279). All these results demonstrated that the C12Im-Cl-assisted sample preparation method is of great promise to promote the large-scale membrane proteome profiling.
语种英语
WOS记录号WOS:000340081100057
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/144459
专题中国科学院大连化学物理研究所
通讯作者Zhang LH(张丽华)
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
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GB/T 7714
Zhao, Qun,Fang, Fei,Liang, Yu,et al. 1-Dodecyl-3-Methylimidazolium Chloride-Assisted Sample Preparation Method for Efficient Integral Membrane Proteome Analysis[J]. ANALYTICAL CHEMISTRY,2014,86(15):7544-7550.
APA Zhao, Qun.,Fang, Fei.,Liang, Yu.,Yuan, Huiming.,Yang, Kaiguang.,...&张丽华.(2014).1-Dodecyl-3-Methylimidazolium Chloride-Assisted Sample Preparation Method for Efficient Integral Membrane Proteome Analysis.ANALYTICAL CHEMISTRY,86(15),7544-7550.
MLA Zhao, Qun,et al."1-Dodecyl-3-Methylimidazolium Chloride-Assisted Sample Preparation Method for Efficient Integral Membrane Proteome Analysis".ANALYTICAL CHEMISTRY 86.15(2014):7544-7550.
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