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学科主题: 物理化学
题名: Epitope imprinted polymer coating CdTe quantum dots for specific recognition and direct fluorescent quantification of the target protein bovine serum albumin
作者: Yang, Ya-Qiong1, 2, 3;  He, Xi-Wen1, 2, 3;  Wang, Yi-Zhi1, 2, 3;  Li, Wen-You1, 2, 3;  Zhang, Yu-Kui1, 2, 3, 4
通讯作者: 李文友
关键词: Epitope imprinting ;  Bovine serum albumin ;  Quantum dots ;  Fluorescent quantification
刊名: BIOSENSORS & BIOELECTRONICS
发表日期: 2014-04-15
DOI: 10.1016/j.bios.2013.11.004
卷: 54, 期:1, 页:266-272
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biophysics ;  Biotechnology & Applied Microbiology ;  Chemistry, Analytical ;  Electrochemistry ;  Nanoscience & Nanotechnology
研究领域[WOS]: Biophysics ;  Biotechnology & Applied Microbiology ;  Chemistry ;  Electrochemistry ;  Science & Technology - Other Topics
英文摘要: A novel epitope molecularly imprinted polymer (EMIP) for specific recognition and direct fluorescent quantification of the target protein bovine serum albumin (BSA) was demonstrated where polymerization was performed on the surface of silica nanospheres embedded CdTe quantum dots (QDs). The synthetic peptide derived from the surface-exposed C-terminus of bovine serum albumin (BSA, residues 599-607) was selected as the template molecule. The resulting EMIP film was able to selectively capture the template peptide and the corresponding target protein BSA via the recognition cavities. Based on the fluorescence quenching, the EMIP-coated QDs (molecular imprinted polymer coating CdTe QDs using epitope as the template) nanospheres were successfully applied to the direct fluorescence quantification of BSA. Compared with BMIP-coated QDs (molecular imprinted polymer coating CdTe QDs using BSA as the template), the imprinting factor and adsorption capacity of EMIP-coated QDs were greatly increased. The prepared EMIP-coated QDs can also discriminate even one mismatched sequences from the original sequences of the epitope of the BSA. The practical analytical performance of the EMIP-coated QDs was examined by evaluating the detection of BSA in the bovine calf serum sample with satisfactory results. In addition, the resulting EMIP-coated QDs nanospheres were also successfully applied to separating BSA from the bovine blood sample. (C) 2013 Elsevier B.V. All rights reserved.
关键词[WOS]: AQUEOUS-MEDIA ;  NANOPARTICLES ;  ANTIBODIES ;  COMPOSITE ;  PEPTIDES
语种: 英语
WOS记录号: WOS:000333071500042
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/144461
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
2.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
3.Synerget Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China

Recommended Citation:
Yang, Ya-Qiong,He, Xi-Wen,Wang, Yi-Zhi,et al. Epitope imprinted polymer coating CdTe quantum dots for specific recognition and direct fluorescent quantification of the target protein bovine serum albumin[J]. BIOSENSORS & BIOELECTRONICS,2014,54(1):266-272.
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