DICP OpenIR
Subject Area物理化学
Epitope imprinted polymer coating CdTe quantum dots for specific recognition and direct fluorescent quantification of the target protein bovine serum albumin
Yang, Ya-Qiong1,2,3; He, Xi-Wen1,2,3; Wang, Yi-Zhi1,2,3; Li, Wen-You1,2,3; Zhang, Yu-Kui1,2,3,4; Li WY(李文友)
KeywordEpitope Imprinting Bovine Serum Albumin Quantum Dots Fluorescent Quantification
Source PublicationBIOSENSORS & BIOELECTRONICS
2014-04-15
DOI10.1016/j.bios.2013.11.004
Volume54Issue:1Pages:266-272
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS Research AreaBiophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS KeywordAQUEOUS-MEDIA ; NANOPARTICLES ; ANTIBODIES ; COMPOSITE ; PEPTIDES
AbstractA novel epitope molecularly imprinted polymer (EMIP) for specific recognition and direct fluorescent quantification of the target protein bovine serum albumin (BSA) was demonstrated where polymerization was performed on the surface of silica nanospheres embedded CdTe quantum dots (QDs). The synthetic peptide derived from the surface-exposed C-terminus of bovine serum albumin (BSA, residues 599-607) was selected as the template molecule. The resulting EMIP film was able to selectively capture the template peptide and the corresponding target protein BSA via the recognition cavities. Based on the fluorescence quenching, the EMIP-coated QDs (molecular imprinted polymer coating CdTe QDs using epitope as the template) nanospheres were successfully applied to the direct fluorescence quantification of BSA. Compared with BMIP-coated QDs (molecular imprinted polymer coating CdTe QDs using BSA as the template), the imprinting factor and adsorption capacity of EMIP-coated QDs were greatly increased. The prepared EMIP-coated QDs can also discriminate even one mismatched sequences from the original sequences of the epitope of the BSA. The practical analytical performance of the EMIP-coated QDs was examined by evaluating the detection of BSA in the bovine calf serum sample with satisfactory results. In addition, the resulting EMIP-coated QDs nanospheres were also successfully applied to separating BSA from the bovine blood sample. (C) 2013 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000333071500042
Citation statistics
Cited Times:84[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/144461
Collection中国科学院大连化学物理研究所
Corresponding AuthorLi WY(李文友)
Affiliation1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China
2.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
3.Synerget Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Yang, Ya-Qiong,He, Xi-Wen,Wang, Yi-Zhi,et al. Epitope imprinted polymer coating CdTe quantum dots for specific recognition and direct fluorescent quantification of the target protein bovine serum albumin[J]. BIOSENSORS & BIOELECTRONICS,2014,54(1):266-272.
APA Yang, Ya-Qiong,He, Xi-Wen,Wang, Yi-Zhi,Li, Wen-You,Zhang, Yu-Kui,&李文友.(2014).Epitope imprinted polymer coating CdTe quantum dots for specific recognition and direct fluorescent quantification of the target protein bovine serum albumin.BIOSENSORS & BIOELECTRONICS,54(1),266-272.
MLA Yang, Ya-Qiong,et al."Epitope imprinted polymer coating CdTe quantum dots for specific recognition and direct fluorescent quantification of the target protein bovine serum albumin".BIOSENSORS & BIOELECTRONICS 54.1(2014):266-272.
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