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学科主题: 物理化学
题名: Metabolomics Study of Hepatocellular Carcinoma: Discovery and Validation of Serum Potential Biomarkers by Using Capillary Electrophoresis-Mass Spectrometry
作者: Zeng, Jun1;  Yin, Peiyuan1;  Tan, Yexiong2;  Dong, Liwei2;  Hu, Chunxiu1;  Huang, Qiang1;  Lu, Xin1;  Wang, Hongyang2;  Xu, Guowang1
通讯作者: HongyangWang ;  许国旺
关键词: hepatocellular carcinoma ;  HCC ;  metabolomics ;  serum ;  biomarkers ;  capillary electrophoresis ;  mass spectrometry
刊名: JOURNAL OF PROTEOME RESEARCH
发表日期: 2014-07-01
DOI: 10.1021/pr500390y
卷: 13, 期:7, 页:3420-3431
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Hepatocellular carcinoma (HCC) is one of the most lethal malignancies. The lack of effective screening methods for early diagnosis has been a longstanding bottleneck to improve the survival rate. In the present study, a capillary electrophoresis-time-of-flight mass spectrometry (CE-TOF/MS)-based metabolomics method was employed to discover novel biomarkers for HCC. A total of 183 human serum specimens (77 sera in discovery set and 106 sera in external validation set) were enrolled in this study, and a "serum biomarker model" including tryptophan, glutamine, and 2-hydroxybutyric acid was finally established based on the comprehensive screening and validation workflow. This model was evaluated as an effective tool in that area under the receiver operating characteristic curve reached 0.969 in the discovery set and 0.99 in the validation set for diagnosing HCC from non-HCC (health and cirrhosis). Furthermore, this model enabled the discrimination of small HCC from precancer cirrhosis with an AUC of 0.976, highlighting the potential of early diagnosis. The biomarker model is effective for those a-fetoprotein (AFP) false-negative and false-postive subjects, indicating the complementary function to conventional tumor marker AFP. This study demonstrates the promising potential of CE-MS-based metabolomics approach in finding biomarkers for disease diagnosis and providing special insights into tumor metabolism.
关键词[WOS]: INDOLEAMINE 2,3-DIOXYGENASE ;  ARGININE DEPRIVATION ;  GLUTAMINE-METABOLISM ;  TUMOR-CELLS ;  CANCER ;  DEPLETION ;  GROWTH ;  PROLIFERATION ;  INHIBITION ;  DIAGNOSIS
语种: 英语
WOS记录号: WOS:000338693400026
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/144483
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Second Mil Med Univ, Int Cooperat Lab Signal Transduct, Eastern Hepatobiliary Surg Inst, Shanghai 116023, Peoples R China

Recommended Citation:
Zeng, Jun,Yin, Peiyuan,Tan, Yexiong,et al. Metabolomics Study of Hepatocellular Carcinoma: Discovery and Validation of Serum Potential Biomarkers by Using Capillary Electrophoresis-Mass Spectrometry[J]. JOURNAL OF PROTEOME RESEARCH,2014,13(7):3420-3431.
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