DICP OpenIR
Subject Area物理化学
Metabolomics Study of Hepatocellular Carcinoma: Discovery and Validation of Serum Potential Biomarkers by Using Capillary Electrophoresis-Mass Spectrometry
Zeng, Jun1; Yin, Peiyuan1; Tan, Yexiong2; Dong, Liwei2; Hu, Chunxiu1; Huang, Qiang1; Lu, Xin1; Wang, Hongyang2; Xu, Guowang1; HongyangWang; Xu GW(许国旺)
KeywordHepatocellular Carcinoma Hcc Metabolomics Serum Biomarkers Capillary Electrophoresis Mass Spectrometry
Source PublicationJOURNAL OF PROTEOME RESEARCH
2014-07-01
DOI10.1021/pr500390y
Volume13Issue:7Pages:3420-3431
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemical Research Methods
WOS Research AreaBiochemistry & Molecular Biology
WOS KeywordINDOLEAMINE 2,3-DIOXYGENASE ; ARGININE DEPRIVATION ; GLUTAMINE-METABOLISM ; TUMOR-CELLS ; CANCER ; DEPLETION ; GROWTH ; PROLIFERATION ; INHIBITION ; DIAGNOSIS
AbstractHepatocellular carcinoma (HCC) is one of the most lethal malignancies. The lack of effective screening methods for early diagnosis has been a longstanding bottleneck to improve the survival rate. In the present study, a capillary electrophoresis-time-of-flight mass spectrometry (CE-TOF/MS)-based metabolomics method was employed to discover novel biomarkers for HCC. A total of 183 human serum specimens (77 sera in discovery set and 106 sera in external validation set) were enrolled in this study, and a "serum biomarker model" including tryptophan, glutamine, and 2-hydroxybutyric acid was finally established based on the comprehensive screening and validation workflow. This model was evaluated as an effective tool in that area under the receiver operating characteristic curve reached 0.969 in the discovery set and 0.99 in the validation set for diagnosing HCC from non-HCC (health and cirrhosis). Furthermore, this model enabled the discrimination of small HCC from precancer cirrhosis with an AUC of 0.976, highlighting the potential of early diagnosis. The biomarker model is effective for those a-fetoprotein (AFP) false-negative and false-postive subjects, indicating the complementary function to conventional tumor marker AFP. This study demonstrates the promising potential of CE-MS-based metabolomics approach in finding biomarkers for disease diagnosis and providing special insights into tumor metabolism.
Language英语
WOS IDWOS:000338693400026
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/144483
Collection中国科学院大连化学物理研究所
Corresponding AuthorHongyangWang; Xu GW(许国旺)
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Second Mil Med Univ, Int Cooperat Lab Signal Transduct, Eastern Hepatobiliary Surg Inst, Shanghai 116023, Peoples R China
Recommended Citation
GB/T 7714
Zeng, Jun,Yin, Peiyuan,Tan, Yexiong,et al. Metabolomics Study of Hepatocellular Carcinoma: Discovery and Validation of Serum Potential Biomarkers by Using Capillary Electrophoresis-Mass Spectrometry[J]. JOURNAL OF PROTEOME RESEARCH,2014,13(7):3420-3431.
APA Zeng, Jun.,Yin, Peiyuan.,Tan, Yexiong.,Dong, Liwei.,Hu, Chunxiu.,...&许国旺.(2014).Metabolomics Study of Hepatocellular Carcinoma: Discovery and Validation of Serum Potential Biomarkers by Using Capillary Electrophoresis-Mass Spectrometry.JOURNAL OF PROTEOME RESEARCH,13(7),3420-3431.
MLA Zeng, Jun,et al."Metabolomics Study of Hepatocellular Carcinoma: Discovery and Validation of Serum Potential Biomarkers by Using Capillary Electrophoresis-Mass Spectrometry".JOURNAL OF PROTEOME RESEARCH 13.7(2014):3420-3431.
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