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学科主题物理化学
Study of polar metabolites in tobacco from different geographical origins by using capillary electrophoresis-mass spectrometry
Zhao, Jieyu; Hu, Chunxiu; Zeng, Jun; Zhao, Yanni; Zhang, Junjie; Chang, Yuwei; Li, Lili; Zhao, Chunxia; Lu, Xin; Xu, Guowang; Xu GW(许国旺)
关键词Capillary Electrophoresis Mass Spectrometry Plant Metabolomics Method Validation Tobacco Leaf
刊名METABOLOMICS
2014-10-01
DOI10.1007/s11306-014-0631-4
10期:5页:805-815
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Endocrinology & Metabolism
研究领域[WOS]Endocrinology & Metabolism
关键词[WOS]SOLID-PHASE MICROEXTRACTION ; FALSE DISCOVERY RATE ; LIQUID-CHROMATOGRAPHY ; GAS-CHROMATOGRAPHY ; CE-MS ; METABOLOMICS ; EXTRACTION ; PLANT ; LEAVES ; OPTIMIZATION
英文摘要Many metabolites in plant are highly polar and ionic. Their analysis using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry can be problematic. Therefore a capillary electrophoresis-mass spectrometry (CE-MS) method with charge-driven separation characteristic was developed to investigate polar metabolites in tobacco. To obtain as many features as possible, extraction of polar metabolites was optimized by the design of experiments and evaluated by univariate statistics. Method validation was carried out to evaluate the analytical characteristics including calibration curve, precision, sample stability and extraction reproducibility. The developed method was successfully applied in studying 30 tobacco leaves obtained from Yunnan and Guizhou provinces in China. A total of 154 polar metabolites were identified based on available database. Multivariate pattern recognition clearly revealed the metabolic differences between the two geographic areas and 43 significantly different metabolites were defined by the non-parametric hypothesis test (Mann-Whitney U test) and false discovery rate. Some key metabolites involved in photosynthesis such as ribulose 1,5-disphosphate, fructose 1,6-diphosphate, glycine, betaine, GABA and serine were found to be susceptible to environmental conditions. This study shows that the metabolic profiling based on CE-MS can clearly discriminate tobacco leaves of different geographical origins and understand the relationship between plant metabolites and their geographical origins.
语种英语
WOS记录号WOS:000341422600004
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/144497
专题中国科学院大连化学物理研究所
通讯作者Xu GW(许国旺)
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
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GB/T 7714
Zhao, Jieyu,Hu, Chunxiu,Zeng, Jun,et al. Study of polar metabolites in tobacco from different geographical origins by using capillary electrophoresis-mass spectrometry[J]. METABOLOMICS,2014,10(5):805-815.
APA Zhao, Jieyu.,Hu, Chunxiu.,Zeng, Jun.,Zhao, Yanni.,Zhang, Junjie.,...&许国旺.(2014).Study of polar metabolites in tobacco from different geographical origins by using capillary electrophoresis-mass spectrometry.METABOLOMICS,10(5),805-815.
MLA Zhao, Jieyu,et al."Study of polar metabolites in tobacco from different geographical origins by using capillary electrophoresis-mass spectrometry".METABOLOMICS 10.5(2014):805-815.
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